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Involvement of flavonol synthase/flavanone 3-hydroxylase-like in expression of phytotoxic potential of potato (Solanum sp.)

ABSTRACT: Glycoalkaloids are Solanum bioactive compounds and are involved in allelopathic interactions. To achieve a better understanding of plant–plant interactions, it is essential to deeply examine the trait distribution in a segregating population. In the present paper, we used transcriptomic and metabolomic approaches to recognize the phytotoxic abilities of potato plants originating from a diploid segregating F1 population with particular emphasis on glycoalkaloids in potato groups characterized by a high glycoalkaloid content. In potato F1 individuals, six glycoalkaloids were recognized: solasonine, solamargine, α-solanine, α-chaconine, leptinine I, and leptine II. In the bulk samples characterized by a high total glycoalkaloid content and various phytotoxic potential, high - A’, low - B’ and hormesis - F’, a significant role of the glycoalkaloid composition in the expression of phytotoxic potential was revealed. In particular, leptine II, solasonine and solamargine were present. Based on the RNA-seq analysis of the bulk samples, a flavonol synthase/flavanone 3-hydroxylase-like gene responsible for flavonoid synthesis was upregulated in comparison A’ vs. B’ and A’ vs. F’ (Log2FC=8.30 and 6.40, respectively). The population-level evaluation of phytotoxic potential confirmed a significant negative correlation between total glycoalkaloid content and phytotoxic potential (r=-0.211) but only after correction for total flavonoid content.

ORGANISM(S): Solanum tuberosum

PROVIDER: GSE186482 | GEO | 2021/10/27

REPOSITORIES: GEO

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Project description:Anthocyanins and flavonols are natural compounds that accumulate preferentially in grapevine flowers and fruits. They are among the most abundant flavonoids and play a very important role in grape and wine quality. In particular, they confer and stabilize colour and contribute to other organoleptic characteristics of the final product. Their complex profile in terms of concentration and relative abundance varies among cultivars and makes wine typicity. The synthesis of these compounds is mainly regulated at transcriptional level. Flavonoids accumulate at specific stages and in specific tissues during flower and berry development, as a consequence of the timely expression of the genes necessary for their synthesis. Although the general flavonoid pathway has been genetically and biochemically elucidated and the main determinants of colour have been identified, the molecular reasons of the fine variation among grape cultivars are still not completely understood. To shed light on this issue, extreme genotypes of a segregating population derived from the cross Syrah x Pinot Noir were characterized at transcriptional level. The transcriptome analysis along three berry developmental stages of these genotypes has allowed the identification of a large set of transcripts differentially modulated between the two groups. A population derived from M-bM-^@M-^XSyrahM-bM-^@M-^Y x M-bM-^@M-^XPinot NoirM-bM-^@M-^Y consisting of 170 F1 individuals plus the parental lines was characterized for the content of flavonols and anthocyanins in the skins of mature berries (38E-L; 18M-BM-0Brix). Based on the biochemical data, 4 high- and 4 low-flavonol producers (HFPs and LFPs: F1 16, F1 56, F1 63, F1 223 and F1 64, F1 256, F1 260, PN), two of which having also either very high or very low anthocyanin content (HAPs and LAPs: F1 63, F1 223 and F1 256, F1 260), were selected for gene expression analysis. A representative sample for each individual (one biological replicate) was collected at three berry developmental stages (hard green berry (33E-L, PV), veraison (35E-L, 50% coloured berries, VER) and maturity (38E-L, 18M-BM-0Brix, MAT)) during the 2007 season.

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Involvement of flavonol synthase/flavanone 3-hydroxylase-like in expression of phytotoxic potential of potato (Solanum sp.)

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