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Differential expression of mitosis and cell cycle regulatory genes during recovery from an acute respiratory virus infection

ABSTRACT: Acute respiratory virus infections can have profound and long-term effects on lung function that persist even after the acute responses have fully resolved. In this study, we examined gene expression by RNA sequencing in lung tissue of wild-type BALB/c mice that are recovering from a sublethal infection with pneumonia virus of mice (PVM) a natural rodent pathogen of the same virus family and genus as human respiratory syncytial virus. We compared these responses to gene expression in PVM-infected mice treated with Lactobacillus plantarum, an immunobiotic agent that limits inflammation and averts the negative clinical sequelae typically observed in response to acute infection with this pathogen. Our findings revealed prominent differential expression of inflammation-associated as well as numerous genes and gene families implicated in mitosis and cell-cycle regulation, including cyclins, cyclin-dependent kinases, cell division cycle genes, E2F transcription factors, kinesins, centromere proteins, and aurora kinases, among others. Of particular note was the differential expression of the cell division cycle gene Cdc20b, which was previously identified as critical for the differentiation of multi-ciliated cells ex vivo. Collectively, these findings provide us with substantial insight into post-viral repair processes and broaden our understanding of the mechanisms underlying Lactobacillus-mediated protection.

ORGANISM(S): Mus musculus

PROVIDER: GSE186740 | GEO | 2021/12/29

REPOSITORIES: GEO

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