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RNA sequencing of RAW264.7 with or without Sal-B and LPS addition

ABSTRACT: Autologous fat grafting (AFG), although an appealing approach to repair soft tissue defects, has various complications. Excessive inflammation at the transplant site is one of the main reasons for the poor effect of fat transplantation and occurrence of complications. Our previous study proved that Salvia miltiorrhiza can enhance fat graft survival. Salvianolic acid B (Sal-B) is the most abundant and bioactive water-soluble compound in Salvia miltiorrhiza and has anti-inflammatory effects on other diseases. Therefore, we hypothesized that salvianolic acid B could improve the effect of fat grafts by inhibiting inflammation.

ORGANISM(S): Mus musculus

PROVIDER: GSE186886 | GEO | 2023/09/14

REPOSITORIES: GEO

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RNA sequencing of adipose tissue-derived stromal cells (ADSCs) with or without Sal-B addition

Project description:Salvianolic acid B (Sal-B) is the most abundant and bioactive water-soluble compound in Salvia miltiorrhiza. Studies have shown that salvianolic acid B increases the mRNA expression of adipogenic transcription factors, including PPARγ, C/EBPα and PPARα, in 3T3-L1 preadipocytes to increase glucose uptake and mitochondrial respiration, reduce glycerol release and promote adipocyte differentiation. However, the effect of Sal-B on graft fat survival and its specific mechanism have not been investigated. So we dicided using h-ADSCs to see its transcriptomes changing after Sal-B addition.

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RNA sequencing of HDFs(Human dermal fibroblasts) with or without Sal-B and UVB treatment

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