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Impact of knocking out Ppargc1a in intestinal stem cells on mucosal homeostasis

ABSTRACT: We report the application of single cell RNAseq assay in examining the effect of Ppargc1a gene expression in intestinal stem cells on mucosal homeostasis. Lgr5EGFPcreERT2 mice were crossed with Ppargc1a F/+ or F/F to selectively target gene expression in Lgr5 expressing intestinal stem cells. Mice were fed with control diet AIN76A from weaning for 3 months, and deletion of Ppargc1a gene was conducted via Tamoxifen injection. Mice were sacrificed 3 days post tamoxifen injection.

ORGANISM(S): Mus musculus

PROVIDER: GSE188339 | GEO | 2023/04/12

REPOSITORIES: GEO

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Impact of knocking out Ppargc1a in intestinal stem cells on mucosal homeostasis

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