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MRNA translation efficiency analysis in liver at 0 h and 24 h after 70% partial hepatectomy

ABSTRACT: In order to detect changes in liver tissue protein translation efficiency during liver regeneration, we performed sucrose density gradient centrifugation to separate translated (associated with polysome fraction), and non-translated (associated with sub-polysome fraction) transcripts in extracts from mouse livers 0 h and 24 h post-surgery. We found that compared to liver tissues before surgery, extracts from tissues 24 h post-surgery had significantly fewer transcripts associated with polysomes, suggesting a reduction in protein synthesis activity.

ORGANISM(S): Mus musculus

PROVIDER: GSE188768 | GEO | 2022/12/30

REPOSITORIES: GEO

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