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Translational profiling of HEK293 cells exposed to cold shock (32 degrees C) and untreated (37 degrees C)

ABSTRACT: This experiment investigates the effect of mild cold-shock (32 degrees C, 24 hours) on the translatome of human HEK293 cells. After cold-shock, cells were treated with cycloheximide to block elongation, then sucrose density gradients were used to separate post-nuclear extracts into polysome and subpolysome fractions. The pooled polysomal fraction was labelled with Cy5, the pooled subpolysomal fraction was labelled with Cy3, and both were hybridised to the same array.

ORGANISM(S): Homo sapiens

SUBMITTER: Amandine Bastide

PROVIDER: E-MTAB-5437 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

RTN3 Is a Novel Cold-Induced Protein and Mediates Neuroprotective Effects of RBM3.

Bastide Amandine A Peretti Diego D Knight John R P JR Grosso Stefano S Spriggs Ruth V RV Pichon Xavier X Sbarrato Thomas T Roobol Anne A Roobol Jo J Vito Davide D Bushell Martin M von der Haar Tobias T Smales C Mark CM Mallucci Giovanna R GR Willis Anne E AE

Current biology : CB 20170223 5

Cooling and hypothermia are profoundly neuroprotective, mediated, at least in part, by the cold shock protein, RBM3. However, the neuroprotective effector proteins induced by RBM3 and the mechanisms by which mRNAs encoding cold shock proteins escape cooling-induced translational repression are unknown. Here, we show that cooling induces reprogramming of the translatome, including the upregulation of a new cold shock protein, RTN3, a reticulon protein implicated in synapse formation. We report th ...[more]

PMID: 28238655

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