Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of IP6K2-deletion on mRNA expression in mouse spinal cord and duodenum

ABSTRACT: Inositol pyrophosphates (PP-IPs) regulate diverse physiological processes. Mammalian genome encodes three enzymes (IP6K1-3) synthesizing PP-IP diphosphoinositol pentakisphosphate (IP7). The objective of this experiment is to elucidate the physiological roles of IP6K2-dependent PP-IPs metabolism in gene expression of central nervous system and gastrointestinal tract. Mouse GE 4×44K v2 microarray was employed to comprehensively analyze mRNA expression in spinal cord and duodenum. We observed that mRNAs prominently increased or decreased by IP6K2 deletion were different in each organ, suggesting differential regulatory roles of IP6K2-IP7 axis in these two organs.

ORGANISM(S): Mus musculus

PROVIDER: GSE189239 | GEO | 2024/11/19

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Role of pp-ips in rpd3 complex and the environmental stress response (ESR)

Project description:Cells respond to stress and starvation by adjusting their growth rate and enacting stress defense programs. In eukaryotes this involves inactivation of TORC1, which in turn triggers downregulation of ribosome and protein synthesis genes and upregulation of stress response genes. Here we report that the highly conserved inositol pyrophosphate second messengers (including 1-PP-IP5, 5-PP-IP4, and 5-PP-IP5) are also critical regulators of cell growth and the general stress response, acting in parallel to the TORC1 pathway to control the activity of the class I HDAC Rpd3L. In fact, yeast cells that cannot synthesize any of the PP-IPs mount little to no transcriptional response in osmotic, heat, or oxidative stress. Furthermore, PP-IP dependent regulation of Rpd3L occurs independently of the role individual PP-IPs (such as 5-PP-IP5) play in activating specialized stress/starvation response pathways. Thus, the PP-IP second messengers simultaneously activate and tune the global response to stress and starvation signals. 2-condition experiments. Includes the responses of wild-type (ACY 044) and mutant yeast strains (all are W303 background) to log growth and stress conditions. This series of microarrays were performed on null mutants of various genes in the inositol pyrophosphate synthesis pathway, including several members of the Rpd3L histone deacetylase complex. All mutants were made in W303 strain, MatA yeast, using standard techniques (homologous recombination). Several stress conditions were tested, including heat-shock, oxidative (H2O2), and osmotic stress (0.375M KCl). Cells in mid-log growth were subjected to stress for 20 minutes. In one instance the TOR inhibitor rapamycin was added to determine whether PP-IPs act above/at or below TORC1 in activating the ESR. Taken together, these microarrays show the role of the inositol pyrophosphate synthesis pathway in activating the ESR in stress.

2013-03-22 | E-GEOD-45370 | biostudies-arrayexpress

Role of pp-ips in rpd3 complex and the environmental stress response (ESR)

2013-03-22 | GSE45370 | GEO

insulin resistant duodenum vs. non-insulin resistant duodenum

Project description:insulin resistant duodenum vs. non-insulin resistant duodenum

| PRJNA615363 | ENA

insulin resistant duodenum vs. non-insulin resistant duodenum

Project description:Transcriptional profiling of duodenum from non-obese patients and patients with morbid obesity comparing non-insulin resistance vs. insulin resistande. Goal was to determine the involvement of the duodenum in the development of insulin resistance and the possible influence of obesity.

2022-06-01 | GSE147562 | GEO

Rat duodenum during immobilization

Project description:In the present study, we examined duodenal gene expression in rats subjected to immobilization, to elucidate the mechanism of the stress response in the duodenum. Keywords: stress response

2007-03-09 | GSE4426 | GEO

miRNA profile of duck duodenum mucosa damage caused by oxidative stress

Project description:We deep sequenced and analyzed miRNAs using deep RNA sequencing (RNA-seq) in cage rearing and traditional breeding duck's duodenum sample of Nonghu NO.2 duck. 21 differentially expressed miRNA were identified in the duodenum. 6 miRNAs were upregulated and 15 were downregulated in the cage rearing duck's duodenum of the Nonghu NO.2 duck compared to their expression in the control group. These findings provided insights into the expression profiles of miRNAs in duck duodenum, and deepened our understanding of miRNAs in oxidative injury of duck.

2023-07-23 | GSE237634 | GEO

RNA-seq in mouse duodenum

Project description:RNA-seq in mouse duodenum

| PRJNA595505 | ENA

Transcriptome analysis of cryptococcal IPK mutants

Project description:In eukaryotes, inositol polyphosphates perform essential metabolic and signaling functions. Using human fungal pathogen Cryptococcus neoformans as a model, we created mutants in three inositol polyphosphates kinases: Arg1, Ipk1 and Kcs1. Each of the mutants produces a unique repertoire of inositol polyphosphates, different from the wild type strain. Comparative phenotypic and transcriptome analyses of wild type and mutant strains indicates that inositol polyphosphate PP-IP5 (IP7) is the key regulator of gene expression, fitness and virulence in C. neoformans.

2016-03-03 | GSE78824 | GEO

Expression data from Chicken duodenum

Project description:The objective of this study was to decipher the molecular basis of feed efficiency in meat-type chicken using duodenum tissues from a chicken population divergently selected for residual feed intake (RFI). Residual feed intake is the deviation of expected feed intake from actual feed intake. Chickens that consume less feed than expected are efficient (LRFI) and chickens that consume more feed than expected are inefficient (HRFI). A divergent selection for RFI was undertaken using an unselected random bred chicken population. RFI at day 35-42 was used as a criterion for selecting low (LRFI) and high (HRFI) RFI. Duodenum tissues were collected from 16 male chickens under sterile conditions experimentation. Tissues were collected from 4 males at days 35 and 42 in each line. Duodenum at 35 and 42 days from a chicken population divergently selected for residual feed intake were utilized for RNA extraction and hybridization on Affymetrix microarrays.

2012-08-01 | E-GEOD-39824 | biostudies-arrayexpress

Expression data from Chicken duodenum

2012-08-02 | GSE39824 | GEO