Dataset Information

Disruption of circadian rhythms by ambient light during neu-rodevelopment leads to autistic-like molecular and behavioral alterations in adult mice

ABSTRACT: Although circadian rhythms are thought to be essential for maintaining body health, the effects of chronic circadian disruption during neurodevelopment remain elusive. Here, using the “Short Day” (SD) mouse model, in which an 8h/8h Light/Dark (LD) cycle was applied during E1-P42, we investigated molecular and behavioral changes after circadian disruption in mice. Adult SD mice fully entrained to the 8h/8h LD cycle and their circadian oscillations of clock protein PERI-OD1 and PERIOD2 were disrupted in the suprachiasmatic nucleus and the hippocampus. RNA-seq revealed changes in the transcriptome associated with neurodevelopment, translational control, and autism. Western blotting and immunostaining identified hyperactivation of the mTOR and MAPK signaling and enhanced global protein synthesis in the hippocampus of SD mice. Electrophysiological recording uncovered enhanced excitatory but attenuated inhibitory synaptic transmission in the hippocampal CA1 pyramidal neurons. These functional changes are corroborated by immature dendritic spine morphology in these neurons. Lastly, autistic-like be-havioral changes, including impaired social interaction and communication, increased repetitive behaviors and impaired novel object recognition and location memory, were found in SD mice. Together, these findings demonstrate autistic-like phenotypes in SD mice and highlight a critical role for circadian rhythm in neurodevelopment.

ORGANISM(S): Mus musculus

PROVIDER: GSE189289 | GEO | 2021/12/29

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Cows exposed to short day photoperiod (SD, 8L:16D) during the 60-day non-lactating period prior to parturition produce more milk in their subsequent lactation compared to cows exposed to long day photoperiod (LD,16L:8D). Although this response is well-established in dairy cows, the underlying mechanisms are not understood. We hypothesized that differential gene expression in cows exposed to SD or LD photoperiods during the dry period could be used to identify the functional basis for the subsequent increase in milk production during lactation. Pregnant, multiparous cows were maintained on a SD or LD photoperiod for 60-days prior to parturition. Mammary biopsies were obtained on days -24 and -9 relative to parturition and Affymetrix GeneChip® Bovine Genome Arrays were used to quantify gene expression. Sixty-four genes were differentially expressed (p ≤ 0.05 and fold-change ≥ |1.5|) between SD and LD treatments. Many of these genes were associated with cell growth and proliferation, or immune function. Ingenuity Pathway Analysis predicted upstream regulators to include TNF, TGFβ1, interferon γ and several interleukins. In addition, expression of 125 genes was significantly different between day -24 and day -9; those genes were associated with milk component metabolism and immune function. The interaction of photoperiod and time affected 32 genes associated with insulin-like growth factor (IGF-I) signaling. Genes differentially expressed in response to photoperiod were associated with mammary development and immune function consistent with the enhancement of milk yield in the ensuing lactation. Our results provide insight into the mechanisms by which photoperiod affects the mammary gland and subsequently lactation. Data were analyzed for the effect of photoperiod treatment (LD minus SD), time relative to parturition (day -24 minus day -9) and the interaction of photoperiod treatment and time ((LD minus SD day -9) minus (LD minus SD day -24))

Dataset Information

Disruption of circadian rhythms by ambient light during neu-rodevelopment leads to autistic-like molecular and behavioral alterations in adult mice

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets