Accumulation of Acetaldehyde in aldh2.1-/- Zebrafish Causes Increased Retinal Angiogenesis and Impaired Glucose Homeostasis
Ontology highlight
ABSTRACT:
ORGANISM(S): Danio rerio
PROVIDER: GSE189416 | GEO | 2022/09/07
REPOSITORIES: GEO
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Project description:Accumulation of Acetaldehyde in aldh2.1-/- Zebrafish Causes Increased Retinal Angiogenesis and Impaired Glucose Homeostasis
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Project description:L. lactis cultures: control vs acetaldehyde-induced
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Project description:Inflammation, including reactive oxygen species and inflammatory cytokines in tissues amplify various post-translational modifications (PTMs) of self-proteins. A number of PTMs have been identified as autoimmune biomarkers in the initiation and progression of Type 1 diabetes (T1D). In this study, we have identified the citrullination of pancreatic glucokinase (GK) as a result of inflammation, triggering autoimmunity and affecting GK biological functions. Glucokinase is expressed in hepatocytes to regulate glycogen synthesis, and in pancreatic beta cells as a glucose sensor to initiate glycolysis and insulin signaling. We identify autoantibodies and autoreactive CD4+ T cells to glucokinase epitopes in the circulation of T1D patients and NOD mice. Finally, citrullination alters GK biologic activity (Km) and suppresses glucose-stimulated insulin secretion. Our studies define glucokinase as a T1D biomarker, providing new insights of how inflammation drives PTMs to create both neoautoantigens and affect beta cell metabolism.
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Project description:Mathematical Definition
symbol/abbreviation
Mathematical symbols
Keq Equilibrium constant
Ki Inhibition constant
Km Affinity constant
v Predicted enzyme activities
Vf Maximal enzyme activities under saturating substrate and activator
conditions, and in the absence of inhibitors
h Hill coefficient
k Rate constant
Abbreviations
ACET Acetaldehyde
ADH Alcohol dehydrogenase
AK Adenylate kinase
ATPcons ATP consuming reactions
bPG 1,3-bisphosphoglycerate
ENO Enolase
ETOHcy Cytoplasmic ethanol
ETOHex Extracellular ethanol
ETOHexp Ethanol transport
GAP Glyceraldehyde 3-phosphate
GAPD Glyceraldehyde 3-phosphate dehydrogenase
GF Glucose facilitator
GK Glucokinase
GLUCcy Cytoplasmic glucose
GLUCex Extracellular glucose
GLUC6P Glucose 6-phosphate
GPD Glucose 6-phosphate dehydrogenase
KDPG 2-keto-3-deoxy-6-phosphogluconate
KDPGA 2-keto-3-deoxy-6-phosphogluconate aldolase
PDC Pyruvate decarboxylase
PEP Phosphoenolpyruvate
PGD 6-phosphogluconate dehydratase
PGK 3-phosphoglycerate kinase
PGL 6-phosphogluconolactonase
PGLACTON 6-phosphogluconolactone
PGLUCONATE 6-phosphogluconate
PGM Phosphoglycerate mutase
P3G 3-phosphoglycerate
P2G 2-phosphoglycerate
PYK Pyruvate kinase
PYR Pyruvate
2020-08-06 | MODEL2008060001 | BioModels