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Stella regulates the development of female germline stem cells by modulating chromatin structure and DNA methylation [MeDIP-seq]

ABSTRACT: Genome-wide DNA methylation was assessed by methylated DNA immunoprecipitation

ORGANISM(S): Mus musculus

PROVIDER: GSE189427 | GEO | 2022/05/25

REPOSITORIES: GEO

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<i>Stella</i> Regulates the Development of Female Germline Stem Cells by Modulating Chromatin Structure and DNA Methylation.

Project description:Female germline stem cells (FGSCs) have the ability to self-renew and differentiate into oocytes. Stella, encoded by a maternal effect gene, plays an important role in oogenesis and early embryonic development. However, its function in FGSCs remains unclear. In this study, we showed that CRISPR/Cas9-mediated knockout of Stella promoted FGSC proliferation and reduced the level of genome-wide DNA methylation of FGSCs. Conversely, Stella overexpression led to the opposite results, and enhanced FGSC differentiation. We also performed an integrative analysis of chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), high-throughput genome-wide chromosome conformation capture (Hi-C), and use of our published epigenetic data. Results indicated that the binding sites of STELLA and active histones H3K4me3 and H3K27ac were enriched near the TAD boundaries. Hi-C analysis showed that Stella overexpression attenuated the interaction within TADs, and interestingly enhanced the TAD boundary strength in STELLA-associated regions. Taking these findings together, our study not only reveals the role of Stella in regulating DNA methylation and chromatin structure, but also provides a better understanding of FGSC development.

| S-EPMC9066111 | biostudies-literature

Stella regulates the development of female germline stem cells by modulating chromatin structure and DNA methylation

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-05-25 | GSE188961 | GEO

Stella regulates the development of female germline stem cells by modulating chromatin structure and DNA methylation [RNA-Seq]

Project description:The expression profile data was obtained by RNA-Seq data.

2022-05-25 | GSE188662 | GEO

Stella regulates the development of female germline stem cells by modulating chromatin structure and DNA methylation [Hi-C_2]

Project description:We use in situ Hi-C to report the most comprehensive survey to date of chromatin organization in female germline stem cells

2022-05-25 | GSE199075 | GEO

Stella regulates the development of female germline stem cells by modulating chromatin structure and DNA methylation [Hi-C]

Project description:We use in situ Hi-C to report the most comprehensive survey to date of chromatin organization in female germline stem cells

2022-05-25 | GSE188661 | GEO

Stella regulates the development of female germline stem cells by modulating chromatin structure and DNA methylation [ChIP-Seq]

Project description:We use ChIP-seq to report the most comprehensive survey to date of chromatin organization in female germline stem cells

2022-05-25 | GSE188660 | GEO

Stella regulates the development of female germline stem cells by modulating chromatin structure and DNA methylation [MeDIP-seq]

Project description:Stella regulates the development of female germline stem cells by modulating chromatin structure and DNA methylation [MeDIP-seq]

| PRJNA782996 | ENA

Stella regulates the development of female germline stem cells by modulating chromatin structure and DNA methylation

Project description:Stella regulates the development of female germline stem cells by modulating chromatin structure and DNA methylation

| PRJNA782994 | ENA

MeDIP

Project description:A Bayesian deconvolution strategy for immunoprecipitation-based DNA methylome analysis

| PRJEB2021 | ENA

MeDIP for ten early passage melanoma cell cultures

Project description:Malignant melanoma is the most fatal skin cancer with a high degree of genetic and epigenetic aberrations. To investigate the role of DNA methylation on melanoma heterogeneity, we performed methylated DNA immunoprecipitation (MeDIP) microarray analysis of 10 primary melanoma cell cultures. methylated DNA immunoprecipitation (MeDIP) microarray analysis of 10 primary melanoma cell cultures

2014-05-28 | E-GEOD-57971 | biostudies-arrayexpress

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