Project description:To search and identify the potential downstream miRNAs of circCpsf6, miRNA sequencing analysis was performed on circCpsf6 silencing models in N2A cells. The differential expression of miRNAs caused by the knockdown of circCpsf6 was revealed in the volcano plot.Heatmaps indicated the upregulated and downregulated clusters of differential miRNAs in N2A cells when circCpsf6 was silenced.
Project description:miRNA-sequencing of grapefruit-derived extracellular vesicles and fusion nanovesicles derived from grapefruit-derived extracellular vesicles and gingival mesenchymal stem cell-derived vesicles. We then performed gene expression profiling analysis to explore the miRNAs derived from grapefruit-derived extracellular vesicles, and the retention rate of miRNAs after membrane fusion
Project description:To explore the unique pathogenesis of DCM and analyzed the differences in gene expression, associated pathways and immune cell infiltration among different organs that are targeted by high glucose by bioinformatics-based strategy. In order to find the specific factors that trigger DCM, we contrasted the gene profile of DCM to that of other diabetic diseases including diabetic peripheral neuropathy (DPN) and nephropathy (DN). we performed RNA-seq and miRNA sequencing on heart tissue from db/db mice to explore the transcriptome alterations in DCM pathogenesis including lncRNA, miRNA and mRNA.
Project description:Purpose: Exosome-derived microRNAs (miRNAs) are potential diagnostic biomarkers. However, little is known about their effectiveness as diagnostic biomarkers of fulminant myocarditis (FM). This study aimed to explore miRNA levels in serum exosomes of patients with FM as potential biomarkers for FM diagnosis. Methods: 10 samples were screened with a exosomal small RNA sequencing platform (RiboBio). A Mann-Whitney test was performed to discover differentially expressed miRNAs in the two pairwise comparisons: FM versus HC. Results: From the differentially expressed miRNAs, fourteen candidate miRNAs discovered via small RNA sequencing with P＜0.05 and fold expression change ＞2 were selected for further testing Conclusions: These data suggested that the miRNA panel in serum-derived exosomes provided excellent diagnostic capability for FM.
Project description:Through the high-throughput sequencing platform illumina Hiseq4000, explore the molecular mechanism of miRNA molecules in the development of gastric cancer
Project description:To explore the potential miRNA‑mRNA regulatory mechanism and relevant signaling pathways, we profiled host miRNA and mRNA levels during T.gondii infection with microarrays, bioinformatics, and integrated genomics analyses. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was performed to validate the reliability of microarray data.The present study performed global expression analysis of 41,000 genes and 2,549 microRNAs using the Agilent Whole Human Genome 8 x 60k slide formats printed using Agilent's 60-mer SurePrint technology, respectively. Compared with the controlled cells, a total of 3,568 mRNAs were significantly differentially expressed, including 1,475 upregulated genes (fold change ≥2) and 463 downregulated genes (fold change ≤0.5).
Project description:MicroRNA (miRNA) dysregulation is well-documented in psychiatric disease, but miRNA dynamics during adolescent and early adult brain maturation, when symptoms first appear for many of these diseases, remain poorly understood. Here, we use RNA sequencing to examine miRNAs and their mRNA targets in cortex and hippocampus from early, mid-, and late adolescent and adult mice. We also use Quantitative Proteomics by tandem mass tag mass spectrometry (TMT-MS) to examine protein dynamics in cortex from the same subjects.
