Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of differently expressed mRNAs in LNPPS overexpression bladder cancer cells

ABSTRACT: In this study, we aimed to explore RNAs profiling regulated by LNPPS in bladder cancer. RNA-seq analysis of three pairs of LNPPS-overexpressing 5637 cells and control cells was completed in this project. A total of 17.6Gb raw data was obtained. In concluion, our data showed that the overexpression of LNPPS caused the significantly upregulation of 82 mRNAs, and the downregulation of 102 mRNAs (|Log2FC|>1.5, p<0.05). Further studies will focus on the potential regulatory mechanisms of these significantly differently expressed mRNAs in the LNPPS-mediated development of bladder cancer.

ORGANISM(S): Homo sapiens

PROVIDER: GSE190917 | GEO | 2023/01/04

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Proteomic analysis of the invasiveness of human bladder cancer cells

Project description:To identification of key drivers involved in the development of muscle invasive bladder cancer, which displays poor prognosis, we isolated one subpopulation of human 5637 bladder cancer cells with highly invasiveness and the other subpopulation of 5637 cells with low invasiveness by Transwell method. Through proteomic analysis, differentially expressed proteins were displayed.

2018-03-15 | PXD007709 | Pride

RNA-Seq analysis of bladder cancer cells after protodioscin treatment

Project description:We performed RNA-seq analysis using different bladder cancer cell lines (5637 and T24) after protodioscin treatment. We reported that protodioscin regulates MAPK pathway-associated genes in 5637 and T24 cells. Protodioscin induces apoptosis and suppresses cell cycle progression, cell migration and invasion in 5637 and T24 cells.

2024-08-30 | GSE212328 | GEO

Transcriptomic analysis of 5637 bladder cancer cell line transfected with MAT1A plamid, 48 hours post transfection

Project description:Transcriptomic analysis of 5637 bladder cancer cell line transfected with MAT1A plamid, 48 hours post transfection

2019-06-22 | GSE133123 | GEO

Proteomic analysis of the invasiveness of human bladder cancer cells

2018-08-27 | MSV000082854 | MassIVE

Chromatin Profiling of 5637 bladder cancer cell line

Project description:In order to investigate epigenetic landscape and potential alterations in bladder, we established the chromatin profiling of 5637 cell line by ChIPseq for the following marks and transcription factor : H3K4me3, H3K9ac, H3K27me3, H3K9me3, H3K27ac, H3K4me1, CTCF and FOXA1.

2023-03-22 | GSE193811 | GEO

Transcription profiling by array of human bladder cancer cell lines with or without TP53 mutations

Project description:The established cell lines RT4, 5637 and T24 from human bladder TCC were obtained from the Cell Bank of the Federal University of Rio de Janeiro, Brazil. The 5637 cells harbor two TP53 mutations, at codon 72 (Arg . Pro) and codon 280 (Arg . Thr).T24 cells contain a TP53 allele encoding an in-frame deletion of tyrosine 126. Both cell lines were established from high-grade bladder tumors. No specific mutations were detected in RT4 cells, which had been established from a low-grade papillary bladder tumor. The RT4 and T24 cell lines were maintained in DulbeccoM-^Rs modified EagleM-^Rs medium (Sigma-Aldrich, Inc, St Louis, MO, USA), and the 5637 cells were kept in Roswell Park Memorial Institute medium (Sigma-Aldrich). Both media were supplemented with 10% fetal bovine serum (Cultilab Ltd, Campinas, Brazil), 100 U/mL penicillin G (Sigma-Aldrich), 100 U/mL streptomycin (Sigma-Aldrich) and 1% kanamycin sulfate (Amresco, Branded Products Group, Solon, OH, USA); cells were cultured at 37M-:C in an atmosphere of 5% CO2. A pooled reference design was chosen. More specifically, each array was hybridized with the same reference sample labeled with Cy5, while the experimental samples (control or treated) were labeled with Cy3.

2010-10-30 | E-MEXP-2859 | biostudies-arrayexpress

Gene expression profile of bladder cancer cell lines treated with Mitomycin C

Project description:Mitomycin C (MMC) is the gold standard treatment for non-muscle invasive bladder cancer (NMIBC). We aimed to evaluate in bladder cancer cell lines (T24, 5637, TCC-SUP and CLS-439) the transcriptomic response to MMC treatment. We used Gene Set Enrichment Analysis (GSEA) to identify biological processes and pathways modulated by MMC treatment.

2021-01-02 | GSE151505 | GEO

Genome-wide DNA methylation maps of Decitabine and/or Cisplatin treated bladder cancer cells.

Project description:It is well established that low-dose Decitabine treatment can sustain long-term antitumor effects by targeting DNA methylation and altering gene expression32. We performed methylated DNA immunoprecipitation (MEDIP) followed by next-generation sequencing in 5637 cells. Daily Decitabine treatment with 100 nM for 72 hr (with or without 100ng/ml cisplatin treatment) reduced the methylation level at CpG-islands in the genome of 5637 cells . However, this low-dose DEC treatment did not cause a significant reduction of DNA methylation in non-CpG islands regions.Our study represents the first detailed analysis of DNA methylome generated by RNA-seq technology in bladder cancer cells treated with low-dose decitabine. This study help to reveal the direct target genes of decitabine in bladder cancer treatement.

2021-04-23 | GSE113537 | GEO

Expression profile of non-muscle invasive (superficial) bladder carcinoma

Project description:Gene expression profiling of 82 human non-muscle invasive bladder carcinoma and 4 normal bladder samples, for a total of 86 tissues. A Cartes d‚àö‚â†Identite des Tumeurs (CIT) study from the 'Institut Curie' and the french 'Ligue Nationale Contre le Cancer' (http://cit.ligue-cancer.net/).

2015-01-01 | E-MTAB-1940 | biostudies-arrayexpress

Identification of differently expressed mRNAs in LNPPS overexpression bladder cancer cells

Project description:Identification of differently expressed mRNAs in LNPPS overexpression bladder cancer cells

| PRJNA788932 | ENA