Role of HB6 in plant development
Ontology highlight
ABSTRACT:
ORGANISM(S): Arabidopsis thaliana
PROVIDER: GSE19095 | GEO | 2010/08/01
SECONDARY ACCESSION(S): PRJNA120519
REPOSITORIES: GEO
Similar Datasets
Project description:rs09-07_hb6 - hb6 - The aim is to determine downstream target of HB6 transcription factor involved in plant development - Transcriptome on Arabidopsis HB6 over-expressing plants and BPM Knock down plants during development Keywords: wt vs mutant comparison 6 dye-swap - CATMA arrays
2010-08-01 | E-GEOD-19095 | biostudies-arrayexpress
Project description:affy_syringae_malaga_athaliana - affy_syringae_malaga_athaliana - - Pseudomonas syringae is a plant pathogenic bacterium that relies on a type III secretion system (T3SS) to cause disease in susceptible hosts and to trigger defence in resistant plants. The T3SS translocates effector proteins directly inside the plant cell. Some P. syringae pathovars translocate the effector HopZ1a, which is recognized in Arabidopsis, triggering the hypersensitive response (HR). This resistance does not depend on the usual defence pathways involved in resistance against other avirulence effectors, so it would be very helpful to know the transcriptomic events that take place in the context of a hypersensitive response triggered by HopZ1a. ULP genes codifies for plant SUMO-proteases. Recent studies by our group revealed that they could be involved in plant defense against microorganisms.-- We infiltrated Arabidopsis thaliana ecotype Columbia wild type with 10mM MgCl2 (as mock inoculation), the virulent pathogen Pseudomonas syringae pv. tomato (Pto), Pto expressing the effector HopZ1a and Pto expressing a HopZ1a derivative carrying a point mutation in a residue essential for the cystein-protease activity. We also grew ulp1c/ulp1d mutant plants to analyze them either without treatment or inoculated with MgCl2 (as mock) and Pto wt. Keywords: gene knock out,normal vs antisens mutant comparison
2011-04-20 | GSE21920 | GEO
Project description:affy_syringae_malaga_athaliana - affy_syringae_malaga_athaliana - - Pseudomonas syringae is a plant pathogenic bacterium that relies on a type III secretion system (T3SS) to cause disease in susceptible hosts and to trigger defence in resistant plants. The T3SS translocates effector proteins directly inside the plant cell. Some P. syringae pathovars translocate the effector HopZ1a, which is recognized in Arabidopsis, triggering the hypersensitive response (HR). This resistance does not depend on the usual defence pathways involved in resistance against other avirulence effectors, so it would be very helpful to know the transcriptomic events that take place in the context of a hypersensitive response triggered by HopZ1a. ULP genes codifies for plant SUMO-proteases. Recent studies by our group revealed that they could be involved in plant defense against microorganisms.-- We infiltrated Arabidopsis thaliana ecotype Columbia wild type with 10mM MgCl2 (as mock inoculation), the virulent pathogen Pseudomonas syringae pv. tomato (Pto), Pto expressing the effector HopZ1a and Pto expressing a HopZ1a derivative carrying a point mutation in a residue essential for the cystein-protease activity. We also grew ulp1c/ulp1d mutant plants to analyze them either without treatment or inoculated with MgCl2 (as mock) and Pto wt. Keywords: gene knock out,normal vs antisens mutant comparison 24 arrays - ATH1
2011-04-20 | E-GEOD-21920 | biostudies-arrayexpress
Project description:rs07-08_gollum - trancript profiling in arabidopsis gollum mutant. Elucidate the role of Gollum gene in the plant development by a RNAi approach. Seeds of WT and RNAi lines were sowed on soil and conserved 2 days at 4°C. The pots were transferred to the greenhouse under long-day conditions (16h light, 22°C). The leaves of one-month old plants were harvested and total RNA were extracted. Keywords: wt vs mutant comparison
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Project description:rs07-08_gollum - trancript profiling in arabidopsis gollum mutant. Elucidate the role of Gollum gene in the plant development by a RNAi approach. Seeds of WT and RNAi lines were sowed on soil and conserved 2 days at 4°C. The pots were transferred to the greenhouse under long-day conditions (16h light, 22°C). The leaves of one-month old plants were harvested and total RNA were extracted. Keywords: wt vs mutant comparison 2 dye-swap - CATMA arrays
2010-05-17 | E-GEOD-13417 | biostudies-arrayexpress
Project description:rs09-03_zf1 - zf1 experiment - Transcriptome analysis of mutants for novel AGO-hook type proteins in Arabidopsis thaliana - Plants were grown on soil in controlled environment under LD (16 h light/8 h dark) and the rosette leaves (8-leaf stage seedlings) were collected for RNA preparation Keywords: normal vs transgenic comparison
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Project description:rs09-08_fie - upregulation of transcripts in the absence of prc2 repressory complex - Role of PRC2 repressory complex for differentiation pathways in Arabidopsis thaliana? - Comparison of transcriptome between two biological replicates of fie mutants in comparison with two biological replicates of Col WT. Keywords: gene knock out
2010-12-01 | GSE19851 | GEO
Project description:The aim of this study is to identify Arabidopsis genes whose expression is altered by aphid feeding. An understanding of the plant aphid interaction at the level of the plant transcriptome will 1) consolidate current areas of investigation focused on the phloem composition (the aphid diet), 2) open up areas of plant aphid interactions for ourselves and other workers, 3) Contribute to understanding the use of new molecular technologies in an environmental context and 4) contribute to existing and development of novel control strategies.Our Arabidopsis/Myzus persicae system provides a valuable model for the study because of: a) the advantages of using Arabidopsis, b) The ability to use clonal insects, c) phloem feeding aphids facilitate focus on a specific cell type, d) aphid stylectomy allows collection of pure phloem sap to monitor ‘phloem phenotype’ of the plant and the insect diet, e) we have techniques to monitor the reproductive performance and feeding behaviour aphids.Our strategy has been to test the function of selected genes, particularly those regulating phloem composition (the feeding site of the aphid) based on current phloem models of phloem function. Gene choice is limited the simplicity of current models of phloem aphid interaction.We propose a simple two treatment (aphid infested vs control plants) experiment that will identify novel target genes for future analysis. Arabidopsis plants (variety Columbia) will be grown in 16/8 light/dark in temperature controlled growth rooms. At growth stage 3.90, when rosette growth is complete, 10 clonal adult Myzus persicae will be caged in clip cages on the two largest leaves on each plant. Control plants will be treated identically except that the cages will be empty. Leaves will be harvested 8 h after infestation. This time point is selected as we know that 90% of aphids are plugged into the sieve element within 2h and that a 6h lag phase has period has previously been used when examining gene expression affected by wounding. In subsequent experiments we will examine time courses of expression of relevant genes using other approaches. Pooling two leaves from each of ten plants will generate the RNA sample, ensuring that expression signals are representative of the population of plants. Keywords: pathogenicity_design
2007-01-22 | GSE6172 | GEO
Project description:rs09-02_quinolinate - quinolinate - Study of the impact of an inductible increase in the NAD concentration in arabidopsis - Comparison between the mutant or the control after a quinolinate treatment Keywords: treated vs untreated comparison,wt vs mutant comparison
2010-07-01 | GSE19084 | GEO
Project description:rs09-08_fie - upregulation of transcripts in the absence of prc2 repressory complex - Role of PRC2 repressory complex for differentiation pathways in Arabidopsis thaliana? - Comparison of transcriptome between two biological replicates of fie mutants in comparison with two biological replicates of Col WT. Keywords: gene knock out 6 dye-swap - CATMA arrays
2010-12-01 | E-GEOD-19851 | biostudies-arrayexpress