T6SS effector induces B. subtilis sporulation mediated by σW
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ABSTRACT: We report the application of a high-throughput technique, RNA-seq, to study the transcriptomic response of Bacillus subtilis growing in the presence of Tse1, a T6SS effector of Pseudomonas chlororaphis
Project description:We report the application of a high-throughput technique, RNA-seq, to study the transcriptomic response of P. chlororaphis and Bacillus subtilis growing in co-cultures in solid medium
Project description:We report the application of a high-throughput technique, RNA-seq, to study the transcriptomic response of P. chlororaphis and Bacillus amyloliquefaciens growing in co-cultures in King B solid medium
Project description:This series of experiments was designed to identify the program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis. The mother cell is one of two cell types generated by asymmetric division of sporulating cells approximately two hours after initiation of sporulation. The program is governed by a hierarchical cascade consisting of the transcription factors: sigmaE, sigmaK, GerE, GerR (YlbO) and SpoIIID. The characterization of the sigmaE regulon was reported in Eichenberger et al. (2003), J. Mol. Biol. 327, 945-972. Here we report the data for sigmaK, GerE, GerR and SpoIIID.
Project description:To obtain global cellular response of Bacillus subtilis WT W168 against the intrinsically produced antimicrobial peptide YydF, we performed RNA-seq experiments. For this, we synthesized YydF, extrinsically added 0.5µM to logarithmic phase growing cells and harvested cells after 10 min exposure. Experiments were performed in triplicates and non-induced Bacillus subtilis WT W168 was used as reference condition.
Project description:To obtain global cellular response of Bacillus subtilis WT W168 when treated with amphotericin B, we performed RNA-seq experiments. For this, we added 10 µg ml-1 amphotericin B to logarithmic phase growing cells and harvested cells after 10 min exposure. Experiments were performed in triplicates and non-induced Bacillus subtilis WT W168 was used as reference condition.
Project description:This series of experiments was designed to identify the program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis. The mother cell is one of two cell types generated by asymmetric division of sporulating cells approximately two hours after initiation of sporulation. The program is governed by a hierarchical cascade consisting of the transcription factors: sigmaE, sigmaK, GerE, GerR (YlbO) and SpoIIID. The characterization of the sigmaE regulon was reported in Eichenberger et al. (2003), J. Mol. Biol. 327, 945-972. Here we report the data for sigmaK, GerE, GerR and SpoIIID.
Project description:The transcriptional control of sporulation in Bacillus subtilis is reasonably well understood, but its translational control is underexplored. Here, we use RNA-seq, ribosome profiling and fluorescence microscopy to study the translational dynamics of B. subtilis sporulation. We identify two events of translation silencing and describe spatiotemporal changes in subcellular localization of ribosomes during sporulation. We investigate the potential regulatory role of ribosomes during sporulation using a strain lacking zinc-independent paralogs of three zinc-dependent ribosomal proteins (L31, L33 and S14). The mutant strain exhibits delayed sporulation, reduced germination efficiency, dysregulated translation of metabolic and sporulation-related genes, and disruptions in translation silencing, particularly in late sporulation. This SuperSeries is composed of the SubSeries listed below.
Project description:We report the application of a high-throughput technique, RNA-seq, to study the transcriptomic response of a Bacillus subtilis mutant for the tasA gene growing in biofilm-inducing solid medium (MSgg).
Project description:To obtain global cellular response of Bacillus subtilis WT W168 when treated with SynAnt 49 (BDTL049) we performed RNA-seq experiments. For this, we added 4 µg ml-1 SynAnt 49 to logarithmic phase growing cells and harvested cells after 10 min exposure. Experiments were performed in triplicates and non-induced Bacillus subtilis WT W168 was used as reference condition.