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Phenotypic skewing of tumor-associated macrophages by cooperative actions of IL-6 and IL-4 in IFN-stimulated differentiating monocytes

ABSTRACT: I IFNs promote differentiating monocytes to differentiate into tumor-associated macrophages (TAMs) and limit the I IFNs anti-tumor effects.But how macrophage polarization is shaped by complex signals in the tumor microenvironment during IFN therapies is incompletely understood. Herein, it was discovered that I IFNs could stimulate transitional monocytes to secrete interleukins IL-4 and IL-6, which interact to influence target genes expression in primary mouse bone-marrow-derived macrophages (BMDMs). To analyze the synergism mechanism, RNA seq was performed. Bone marrow derived macrophages were cultured under the 20ng/ml M-CSF for 7days, and treated with the indicated cytokine, samples were harvested for total RNAs and subjected to RNA sequencing analyses.

ORGANISM(S): Mus musculus

PROVIDER: GSE192371 | GEO | 2022/12/31

REPOSITORIES: GEO

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