Effects of the SLICK1 mutation in PRLR on regulation of core body temperature and global gene expression in liver in cattle
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ABSTRACT:
ORGANISM(S): Bos taurus
PROVIDER: GSE192471 | GEO | 2022/01/04
REPOSITORIES: GEO
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Project description:The SLICK1 mutation confers thermotolerance to cattle inheriting one or two copies of the gene. Results are unclear as to whether the mutation changes capacity of animals to undergo sweating during heat stress. Accordingly, differences in characteristics of sweat glands between slick and wildtype Holstein heifers was determined. There were no differences in the proportion of skin occupied by sweat glands but sweat glands from slick heifers had higher amounts of immunoreactive FOXA1 than wildtype heifers. FOXA1 is a transcription factor important for sweating. While results do not support the idea that the SLICK1 mutation changes the abundance of sweat glands in skin, it did affect functional properties of sweat glands, as indicated by increased abundance of immunoreactive FOXA1.
2023-04-01 | GSE200138 | GEO
Project description:Effects of the SLICK1 mutation in PRLR on regulation of core body temperature and global gene expression in liver in cattle
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Project description:Investigation of whole genome gene expression level changes in a Brucella melitensis delta prlr mutant compared to the wild type strain. The mutants analyzed in this study are further described in A. Mirabella, R-M Yanez, R.M. Delrue, S. Uzureau, M.S. Zygmunt, A. Cloeckaert, X. De Bolle, J.J. Letesson (2012). The two component system PrlS/PrlR of Brucella melitensis is required for persistence in mice and appears to respond to ionic strength. Microbiology A six chip study using total RNA recovered from three separate wild-type cultures of Brucella melitensis 16M and three separate cultures of a prlR mutant strain. Each chip measures the expression level of 3,198 genes from Brucella melitensis 16M with nineteen 60 mer probe pairs (PM/MM) per gene, with three-fold technical redundancy.
2012-12-31 | E-GEOD-34633 | biostudies-arrayexpress
Project description:Investigation of whole genome gene expression level changes in a Brucella melitensis delta prlr mutant compared to the wild type strain. The mutants analyzed in this study are further described in A. Mirabella, R-M Yanez, R.M. Delrue, S. Uzureau, M.S. Zygmunt, A. Cloeckaert, X. De Bolle, J.J. Letesson (2012). The two component system PrlS/PrlR of Brucella melitensis is required for persistence in mice and appears to respond to ionic strength. Microbiology
2012-12-31 | GSE34633 | GEO
Project description:The study is to explore differentially expressed miRNAs in AsPC-1 cells overexpressing Ctrl or PRLR.
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Project description:Mouse models of prolactinoma can be useful to better understand molecular mechanisms involved in abnormal lactotroph cell proliferation and secretion. We have previously developed a prolactin receptor deficient (Prlr -/-) mouse, in which Prlr -/- females develop large secreting prolactinomas from 12 months of age with a penetrance of 100%, mimicking human aggressive densely granulated macroprolactinoma, a highly secreting subtype.
2018-02-21 | GSE100334 | GEO
Project description:ABSTRACT:Pregnancy requires a higher functional beta cell mass and this is associated with profound changes in the gene expression profile of pancreatic islets. Taking Tph1 as a sensitive marker for pregnancy-related islet mRNA expression in female mice, we previously identified prolactin receptors and placental lactogen as key signalling molecules. Since beta cells from male mice also express prolactin receptors, the question arose whether male and female islets have the same phenotypic resilience at the mRNA level during pregnancy. We addressed this question in vitro, by using islet tissue culture with placental lactogen and in vivo, by transplanting male or female islets into female acceptor mice. Additionally, the islet mRNA expression of pregnant prolactin receptor deficient mice was compared with that of their pregnant wild-type littermates. When cultured with placental lactogen, or transplanted in female recipients that became pregnant (day 12.5), male islets induced the ‘islet pregnancy gene signature’, which we defined as the 12 highest induced genes in non-transplanted female islets at day 12.5 of pregnancy. In addition, serotonin immunoreactivity was also induced in these male transplanted islets at day 12.5 of pregnancy. In order to investigate the importance of prolactin receptors in these mRNA changes we used a prolactin receptor deficient mouse model. For the 12 genes of the signature, which are highly induced in control pregnant mice, no significant induction of mRNA transcripts was found at day 9.5 of pregnancy. Together, our results support the key role of placental lactogen as a circulating factor that can trigger the pregnancy mRNA profile in male and female beta cells. Islets were isolated from non-prengant (NP) and pregnant (day 9.5) PRLR+/+ and PRLR-/- mice for RNA extraction and hybridization on Affymetrix microarrays. For every condition 3 biological replicates were used.
2015-03-31 | E-GEOD-59141 | biostudies-arrayexpress
Project description:The study is to explore differentially expressed genes in AsPC-1 cells overexpressing Ctrl or PRLR-SF.
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Project description:The study is to explore differentially expressed genes in SW-1990 cells infected with sh-Ctrl or sh-PRLR.
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Project description:A lower core body temperature set point has been suggested to be a factor that could potentially predispose humans to develop obesity.We tested the hypothesis that obese individuals have lower core temperatures than those in normal-weight individuals.In study 1, nonobese [body mass index (BMI; in kg/m(2)) <30] and obese (BMI ?30) adults swallowed wireless core temperature-sensing capsules, and we measured core temperatures continuously for 24 h. In study 2, normal-weight (BMI of 18-25) and obese subjects swallowed temperature-sensing capsules to measure core temperatures continuously for ?48 h and kept activity logs. We constructed daily, 24-h core temperature profiles for analysis.Mean (±SE) daily core body temperature did not differ significantly between the 35 nonobese and 46 obese subjects (36.92 ± 0.03°C compared with 36.89 ± 0.03°C; P = 0.44). Core temperature 24-h profiles did not differ significantly between 11 normal-weight and 19 obese subjects (P = 0.274). Women had a mean core body temperature ?0.23°C greater than that of men (36.99 ± 0.03°C compared with 36.76 ± 0.03°C; P < 0.0001).Obesity is not generally associated with a reduced core body temperature. It may be necessary to study individuals with function-altering mutations in core temperature-regulating genes to determine whether differences in the core body temperature set point affect the regulation of human body weight. These trials were registered at clinicaltrials.gov as NCT00428987 and NCT00266500.
| S-EPMC3076651 | biostudies-literature