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Single nuclei RNA sequencing of skin draining lymph nodes in the setting of skin inflammation and RAG1 fate mapping

ABSTRACT: Type 2 inflammation contributes to the pathology of skin diseases such as atopic dermatitis (AD) and urticaria. We previously found that AD-like inflammation induced by calcipotriol in mice is critically mediated by group 2 innate lymphoid cells (ILC2s), while T cells and B cells are dispensable given development of robust AD-like disease in RAG knockout mice. Indeed, we found that in RAG knockout mice AD-like inflammation was worse, with a proportion of ILC2s displaying increased markers of activation. The goal of this study was to characterize transcriptional and epigenomic changes in ILC populations with a history, or not, of expressing RAG1 in the setting of AD-like skin inflammation.

ORGANISM(S): Mus musculus

PROVIDER: GSE192597 | GEO | 2024/04/28

REPOSITORIES: GEO

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Comparing transcriptional profiles of type 2 innate lymphoid cells from WT and Rag1 knockout mice

Project description:Type 2 inflammation contributes to the pathology of skin diseases such as atopic dermatitis and urticaria. Type 2 innate lymphoid cells are key mediators of skin inflammation by releasing type 2 cytokines in response to certain environmental stimuli. We recently found that Rag1 knockout mice exhibit more pronounced skin inflammation in a mouse model of atopic-dermatitis-like disease, despite lacking adaptive immune cells like T helper 2 cells, implicating a critical role of type 2 innate lymphoid cells in this condition. The goal of this study was to characterize transcriptional differences between WT and Rag KO type 2 innate lymphoid cells to determine if Rag knockout leads to cell-intrinsic alterations in innate lymphoid cell function.

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Project description:Purpose: To analyze the transcriptome difference of ILC2s isoalted from WT and RAG-/- mice under inflammaed condition Method: Lung ILC2s from mice chronically challenged with papain (40 µg/20 µl PBS, 6 subsequest papain challenges, day 0, 1, 2, 13, 14, and 15)

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Transcription profiling of lesional and non-lesional skin from actopic dermititis patients and normal skin

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Transcription data from Normal Skin and Nonlesional and Lesional Atopic Dermatitis/Eczema Skin

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Der f/SEB-induced skin inflammation

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2013-12-10 | E-GEOD-53132 | biostudies-arrayexpress

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2024-03-18 | E-MTAB-12219 | biostudies-arrayexpress

Microdialysis analysis of common inflammatory skin diseases highlights abnormalities in nonlesional skin of patients with atopic dermatitis

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