Long non-coding RNA sequencing (RiboZero) analysis of monocytes-differentiated macrophages
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ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE192642 | GEO | 2024/12/24
REPOSITORIES: GEO
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| GSE192642_Processed_data_macrophages_LncRNAs.xlsx | Xlsx |
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Raw mRNA data normalization and low intensity filtering: Raw signal intensities were normalized in RMA method by NimbleScan v2.5, and low intensity mRNAs were filtered. (mRNAs that at least 2 out of 4 samples have values greater than or equal to lower cut-off: 50.0 were chosen for further analysis) 2. Quality assessment of mRNA data after filtering: Contains Box Plot and Scatter Plot for mRNAs after filtering. 3. Differentially expressed mRNAs screening: Contains significant differentially expressed mRNAs that passed Fold Change filtering (Fold Change >= 2.0). 4. Heat Map and Hierarchical Clustering: Hierarchical Clustering of the mRNAs after filtering. 5. Pathway analysis: Pathway analysis of the differentially expressed mRNAs. 6. GO analysis: GO term analysis of the differentially expressed mRNAs. 1. Raw lncRNA data normalization and low intensity filtering: Raw signal intensities were normalized in RMA method by NimbleScan v2.5, and low intensity lncRNAs were filtered. 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