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Amphiregulin couples IL1RL1+ regulatory T cells and cancer-associated fibroblasts to inhibit antitumor immunity

ABSTRACT: We demonstrated that IL-33 induced strong CD8+ T cell antitumor immune responses characterized by robust clonal expansion and vigorous functional diversification. Nonetheless, IL-33 also produced intense regulatory T cell (Treg) accumulation in the TME, dominated by the IL1RL1+ Treg subset. We further showed that IL1RL1 signaling in Treg cells greatly dampened the antitumor activity of IL-33.

ORGANISM(S): Mus musculus

PROVIDER: GSE193189 | GEO | 2023/08/30

REPOSITORIES: GEO

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Amphiregulin couples IL1RL1+ regulatory T cells and cancer-associated fibroblasts to inhibit antitumor immunity

Project description:Amphiregulin couples IL1RL1+ regulatory T cells and cancer-associated fibroblasts to inhibit antitumor immunity

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Quantitative phosphoproteomic analysis of IL-33 mediated signaling

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RNA-Sequencing of ST2+ Treg cells in response to IL-33 stimulation

Project description:The effects of IL-33 on ST2+ Treg cells were not studied thouroughly. We FACS-sorted in vitro expanded ST2+ Treg cells from C57BL/6 Foxp3-IRES-mRFP (B6 FIR) mice. We next used RNA-seq techonology to define how recombinant IL-33 (rIL-33) may impact mouse Treg by to assessing the transcriptome of IL-33-stimulated ST2+ Treg cells compared to that of untreated ST2+ Treg cells. Our data revealed that ST2+ Treg stimulated with rIL-33 for 6 hours exhibited increased expression of Il10 and Il13 compared to unstimulated ST2+ Treg cells.

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2016-06-27 | E-MTAB-4677 | biostudies-arrayexpress

The IL-33/ST2 axis and tissue Treg maintains epithelial homeostasis in the skin and restrains cancer development

Project description:IL-33 is constitutively expressed in many epithelial tissues at steady state and signals through the receptor, ST2. IL-33 is released upon tissue injury and functions as an endogenous danger signal to alert the immune system to tissue damage. Here we investigate the physiological role of the IL-33/ST2 axis in skin homeostasis and cancer development. We show that expression of IL-33 differentiates malignant from normal/benign human tissues and that in mouse models of cutaneous squamous cell carcinoma the IL-33/ST2 axis protects against carcinogenesis. Tissue Treg are the predominant cells expressing ST2 in the skin and localise around the hair follicle and IL-33+ epithelial cells (EC). Adoptive transfer experiments demonstrate that skin Treg regulate EC differentiation, minimizes mutational load and retrains cancer development following exposure to an environmental carcinogen. Our findings indicate an important role for direct EC-Treg cross-talk as an early checkpoint to contain tissue damage and carcinogenesis.

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