Project description:Comparison of gene expression, in immature green fruit (15dpa), between transgenic tomato (S. lycopersicum cv. '.T63') lines expressing constitutively the transcription factors AtGLK1 (At2g20570) or AtGLK2 (At5g44190) regulated by the CaMV35S (p35S) to 'T63' lines expressing only the CaMV35S promoter construct (TControl) with no AtGLK sequences. The goal is to describe the effect on fruit transcriptome imposed by the presence of the transgens and relate defined transcriptome changes to the biochemical and morphological phenotypes observed in transgenic fruits
Project description:To prevent activation of plant innate immunity the oomycete pathogen Hyaloperonospora arabidopsidis translocates effector proteins into infected cells of its host Arabidopsis thaliana. We noticed that some H. arabidopsidis effectors, when over-expressed in A. thaliana, render the plant more susceptible to infection by biotrophic pathogens (Fabro et al., 2011, PubMed PMID: 22072967). Here we performed transcriptome profiling of a representative transgenic line constitutively expressing H. arabidopsidis effector HaRxL106. We compared the transcriptomes of A. thaliana wild-type (Col-0) plants and an isogenic line expressing HaRxL106 before pathogen challenge and 24 h after infection with the compatible bacterial pathogen Pseudomonas syringae pv. tomato strain DC3000. HaRxL106 interacts with several Arabidopsis proteins (Mukhtar et al., 2011, PubMed PMID: 21798943; Wirthmueller et al., 2015, PubMed PMID: 25284001). To test whether the HaRxL106-interacting A. thaliana proteins MODIFIER OF SNC1, 6 (MOS6), 6B-INTERACTING PROTEIN 1-LIKE 1 (ASIL1) or RADICAL-INDUCED CELL DEATH1 (RCD1) are altered in their transcriptional response to a biotrophic pathogen we performed transcriptome profiling of mos6-1, asil1-1 and rcd1-1 mutants before and 24 h after infection with P. syringae pv. tomato DC3000.
Project description:To generate an efficient defense against begomovirus, we modulated the activity of the immune defense receptor NIK (NSP-Interacting Kinase) in tomato plants; NIK is a virulence target of the begomovirus NSP during infection. Replacing threonine-474 with aspartate (T474D) within the kinase activation loop promoted the constitutive activation of NIK-mediated defenses. This activation resulted in the down-regulation of translation-related genes and the suppression of global translation in T474D-overexpressing tomato lines. We also found that T474D-induced defense-related transcripts were associated with polysomes and immune proteins, which accumulated to detectable levels in T474D leaves. Consistent with these findings, T474D transgenic lines were tolerant to the tomato-infecting begomoviruses ToYSV and ToSRV. We propose that NIK mediates an anti-viral response via translation suppression and immune system induction. Global variation on gene expression induced by NIK expression and virus infection using total RNA from mock-inoculated and ToYSV-infected tomato wild-type plants, mock-inoculated and infected 35S::NIK1-4 overexpressing lines and mock-inoculated and infected 35S::T474D overexpressing lines. File map_itag23.csv correlates the ITAG 2.3 cDNA ID with the 21 bp reads in file Profiles_with_differential_expressions.csv.
Project description:We found that Arabidopsis plants constitutively expressing OsRR6 exhibit reduced cytokinin sensitivity, adventitious root formation and enhanced anthocyanin accumulation. In addition they are hypersensitive to red, far-red and blue light in hypocotyl growth assay and also flower little early as compared to wild type.Therefore, to identify the downstream pathways affected in the OsRR6 overexpression plants, we performed transcriptome profiling of overexpression line vs wild type using Affymetrix microarray platform.
Project description:To identify the regulatory targets of the R2R3-Myb transcription factor, LjMyb14, the gene was constitutively over-expressed in Lotus japonicus under the Lotus ubiquitin promoter. The gene expression levels of three biological replicates of the Lotus japonicus (MG20) were averaged and compared to the the gene expression levels of three independent lines of Lotus japonicus japonicus constituitively over expressing LjMyb14 using the Lotus ubiquitin promoter.
Project description:To reduce the level of major second messenger inositol-1,4,5-triphosphate (InsP3), we generated transgenic tomato plants (cv. Micro-Tom) that are expressing InsP 5-ptase gene. To understand effects of transgene (InsP 5-ptase) on gene expression we carried out microarray analysis from different parts of transgenic and control (wild type, empty vector control) tomato plants Objectives for this study included the identification of genes that were up or down-regulated at the transcriptional level in transgenic tomato plants expressing InsP 5-ptase gene Keywords: genetic modification
Project description:The tomato SlWRKY3 transcription factor was overexpressed in cultivated tomato (Solanum lycopersicum)and transgenic plants transcriptome was compared to that of wild-type plants.
Project description:The tomato SlDREB2 transcription factor was overexpressed in cultivated tomato (Solanum lycopersicum) and transgenic plants tolerance to salinity was compared to that of wild-type plants.
Project description:Constitutive expression of VvMYBPAR in Arabidopsis was found to accumulate proanthocyanidins when the plants were grown on sucrose-supplemented media to induce anthocyanins. To identify the putative targets of VvMYBPAR, the transcriptome analysis of the transgenic lines which highly express VvMYBPAR was carried out using NimbleGen microarray. Three transgenic lines in which VvMYBPAR were constitutively expressed under the control of 35S promoter vs. empty vector transformants were compared.
Project description:We sought to elucidate the molecular mechanisms whereby LIN28B functions by comparing the gene expression profile of cells constitutively expressing LIN28B to empty vector controls. Accordingly, we performed microarray analysis on total RNA isolated from empty vector LoVo and LIN28B-expressing LoVo colon cancer cell lines.
