Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

DNA microarrays time course of SKO-infected MEFs treated with Vc

ABSTRACT: In order to understand the global gene expression changes resulting from the addition of vitamin C (Vc) to SKO (sox2, klf4 and oct4)-transduced mouse embryonic fibroblasts (MEFs), we used microarray to compare the gene expression profile at different time points with or without Vc.

ORGANISM(S): Mus musculus

PROVIDER: GSE19377 | GEO | 2010/01/31

SECONDARY ACCESSION(S): PRJNA123903

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

DNA microarrays (time course) of MEFs transduced with SKO plus HDAC7 or MEF2C

Project description:Overexpression HDAC7 can enhance iPS efficiency in SKO by supressing MEF2 factors We used microarrays to identify changes induced by overexpression of HDAC7 or MEF2C. MEFs were transduced with SKO plus HDAC7 or MEF2C compared to SKO plus empty vector(Flag), GFP as controls. TRIZOL cell lysates were prepared from D6 and D10.

2011-08-17 | E-GEOD-23692 | biostudies-arrayexpress

DNA microarrays (time course) of MEFs transduced with SKO plus HDAC7 or MEF2C

Project description:Overexpression HDAC7 can enhance iPS efficiency in SKO by supressing MEF2 factors We used microarrays to identify changes induced by overexpression of HDAC7 or MEF2C.

2011-08-18 | GSE23692 | GEO

DNA microarrays time course of a MaF pre-iPSC clone (C9) untreated or treated with Vc

Project description:Treatment with vitamin C (Vc) on MaF pre-induced pluripotent stem cells (pre-iPSCs) induced a rapid conversion into full-iPSCs within a few passages. We used microarrays to identify changes induced by Vc in the MaF pre-iPSC clone.

2010-01-31 | GSE19378 | GEO

DNA microarrays time course of SKO-infected MEFs and MaF pre-iPSCs treated with Vc

Project description:This SuperSeries is composed of the SubSeries listed below.

2010-01-31 | GSE19379 | GEO

DNA microarrays (time course) in the iPS process treated with microRNA clusters

Project description:Treatment with MicroRNA cluster B and C increase the iPS efficiency We used microarrays to identify changes induced by MicroRNA clusters in the iPS process One group were MEFs infected with SKO factors, plus MicroRNA cluster B, C or control blank virus. The other group were MEFs infected with MicroRNA cluster B, C or control blank virus only.TRIZOL cell lysates were prepared at D4 and D8.

2012-04-12 | E-GEOD-23104 | biostudies-arrayexpress

Mus musculus

Project description:DNA microarrays time course of SKO-infected MEFs treated with Vc

| PRJNA123903 | ENA

Genes regulated by Gadd45a during somatic cell reprogramming

Project description:Gadd45a can enhance somatic cell reprogramming significantly. To explore the roles of Gadd45a playing in reprogramming, we performed whole genome microarray to identify genes and signals pathways that regulated by Gadd45a. Genes expression of MEFs was measured at day8 in reprogramming. Three samples were set: MEFs infected with SKO plus Flag, MEFs infected with SKO plus Gadd45a and MEFs infected with SKO plus G39A which is a negative mutant of Gadd45a.

2014-04-22 | E-GEOD-56943 | biostudies-arrayexpress

Histone H3K4 and H3K27 trimethylation analysis in ES cells, Mefs and induced pluriptent cells.

Project description:Mefs were transduced with Oct4 , Sox2, Klf4 and c-myc to induce pluripotency. The histone H3 K4 and H3 K27 trimethylation status was compared genome wide in ES cells, MEFs, and induced pluripotent cells (iPS). Keywords: ChIP-Chip

2007-05-23 | GSE7815 | GEO

Inflammation-Driven Carcinogenesis is Mediated through STING [MEFs]

Project description:Chronic stimulation of innate immune pathways by microbial agents or damaged tissue is known to promote inflammation-driven tumorigenesis by unclarified mechanisms1-3. Here we demonstrate that mutagenic 7,12-dimethylbenz(a)anthracene (DMBA), etoposide or cisplatin induces nuclear DNA leakage into the cytosol to intrinsically activate STING (Stimulator of Interferon Genes) dependent cytokine production. Inflammatory cytokine levels were subsequently augmented in a STING-dependent extrinsic manner by infiltrating phagocytes purging dying cells. Consequently, STING-/- mice, or wild type mice adoptively transferred with STING-/- bone marrow, were almost completely resistant to DMBA-induced skin carcinogenesis compared to their wild type counterparts. Our data emphasizes, for the first time, a role for STING in the induction of cancer, sheds significant insight into the causes of inflammation-driven carcinogenesis, and may provide therapeutic strategies to help prevent malignant disease Total RNA obtained from wild type murine embryonic fibroblasts (WT MEFs), STING deficient MEFs (SKO), Trex1 deficient MEFs (TKO), and both STING and Trex1 deficient MEFs (STKO) treated with DMBA and examined cytokine production by these cells.

2014-05-31 | E-GEOD-57603 | biostudies-arrayexpress

RNA sequencing of wildtype, PTEN single knockout (SKO), and PTEN;Rb1 double knockout (DKO) mouse prostate organoids

Project description:The goal of this study was to perform transcriptomics on wildtype, PTEN single knockout (SKO) and PTEN;Rb1 double knockout (DKO) mouse prostate organoids. We isolated basal cells from PTEN floxed and PTEN;Rb1 floxed mouse prostates and infected with either RFP control or Cre recombinase to establish wildtype, SKO, and DKO mouse prostate organoids.

2023-09-01 | GSE221020 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data