Single nucelus RNA sequencing of 14 week EGA fetal skin
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE195657 | GEO | 2023/02/08
REPOSITORIES: GEO
Similar Datasets
Project description:Wnt-responsive mesenchymal cells in dorsal back skin and ventral foot skin
| PRJNA337944 | ENA
Project description:Transgenic mice over-experssing human KLK5 (Tg.hKLK5) in the epidermis develop an inflammatory skin phenotype resembling Netherton syndrome. To characterize the skin inflammation phenotype of this mouse model at the molecular level, we collected back skin samples from young mice at three weeks of age, when the skin phenotype is most severe.
2024-02-05 | GSE224673 | GEO
Project description:We performed single-cell RNA seq on C57/BL6 mouse back skin at E13.5, E16.5, and P0 to study embryonic hair follicle development. We analyzed 15,086 single cell transcriptome profiles from E13.5, E16.5 and newborn mice (postnatal day 0, P0) dorsal skin cells across hair follicle induction, organogenesis, cytodifferentiation stage. Based on t-distributed Stochastic Neighbor Embedding (tSNE) clustering, we identified 14 cell clusters from skin cells and delineated their cell identity gene expression profile. By using Monocle pseudotime ordering analysis, we constructed epithelium/dermal cell lineage differentiation trajectory and revealed sequential activation of key regulons involved during embryonic hair follicle morphogenesis. Our findings here provide molecular landscape during hair follicle epithelium/dermal cell lineage fate decisions.
2020-07-01 | GSE131498 | GEO
Project description:Dkk4 transgenic mice in Tabby background lack hair follicle formation. Gene expression profiling showed significant down regulation of Shh pathway genes at E16.5 and E17.5, the secondary hair follicle induction stages. Keywords: development or differentiation design,genetic modification design To define target genes of Dkk4 during secondary hair follicle induction, we carried out microarray experiments with mouse back skin samples that from 2 developmental time points including E16.5 and E17.5 of Tabby and Tabby background Dkk4 transgenic mice.
2010-09-03 | E-GEOD-19312 | biostudies-arrayexpress
Project description:Hair follicle (HF) regeneration begins when communication between quiescent epithelial stem cells (SCs) and underlying mesenchymal dermal papillae (DP) generates sufficient activating cues to overcome repressive BMP signals from surrounding niche cells. We uncovered a hitherto unrecognized DP transmitter, TGFβ2, which activates Smad2/3 transiently in HFSCs concomitant with entry into tissue regeneration. We used microarrays to detect the genes specifically affected by TGFß receptor II-deficient mice upon HFSC activation. Hair follicle stem cells (HFSCs) of hair gem (HG) and bulge, and total skin keratinocytes were FACS-purified from the mouse back skin at 2nd telogen-to-anagen transition stages.
2012-01-04 | E-GEOD-33471 | biostudies-arrayexpress
Project description:Global expression analysis of neural crest-like skin-derived precursors (SKPs) and Sox2-positive follicle dermal cells that SKPs originate from. In spite of the remarkable regenerative capacity of mammalian skin, an adult dermal stem cell has not yet been identified. Here, we provide evidence that SKPs, multipotent neural crest-like skin-derived precursors, represent an adult dermal stem cell. When transplanted into adult skin, SKPs can reconstitute the adult dermis, contribute to dermal wound-healing, home to a hair follicle niche, and instruct epidermal cells to make hair follicles. Hair follicle-derived SKPs self-renew, maintain their multipotency, and serially reconstitute hair follicles. The endogenous origin of SKPs are Sox2-positive follicle dermal cells that share a similar global gene expression profile with SKPs. These endogenous cells home back to their follicle niche, induce hair follicle morphogenesis, and differentiate into neural and dermal progeny. Hair follicle-associated dermal cells will move out of their niche to contribute to dermal maintenance and wound-healing. These studies therefore identify a dermal stem cell, and provide a biological rationale for the presence of a multipotent precursor within adult dermis, findings with important therapeutic implications.
2009-10-23 | GSE18690 | GEO
Project description:A permantly active form of the oncogene Akt was expressed in the keratinocytes of the basal proliferative layer of the epidermis. Stem cells of the hair follicle expressing the cell surface marker CD34 were isolated. RNA form the CD34(+) and CD34(-) keratinocytes was extracted and and hybridized to Mouse Genome 430 2.0 Affymetrix arrays. Gene expression was compared between CD34(+) hair follicle stem cells and CD34(-) cells isolates from the back skin of K5-myrAkt transgenic mice.
2014-01-26 | E-GEOD-48522 | biostudies-arrayexpress
Project description:In adult skin, each hair follicle contains a reservoir of stem cells (the bulge), which can be mobilized to regenerate the new follicle with each hair cycle and to reepithelialize epidermis during wound repair. Here we report new methods that permit their clonal analyses and engraftment and demonstrate the two defining features of stem cells, namely self-renewal and multi-potency. We also show that, within the bulge, there are two distinct populations, one of which maintains basal lamina contact and temporally precedes the other, which is suprabasal and arises only after the start of the first postnatal hair cycle. This spatial distinction endows them with discrete transcriptional programs, but surprisingly, both populations are growth inhibited in the niche but can self-renew in vitro and make epidermis and hair when grafted. These findings suggest that the niche microenvironment imposes intrinsic “stemness” features without restricting the establishment of epithelial polarity and changes in gene expression. FACS was used to isolate pure populations of living total K14-GFP-actin expressing cells and K14-GFP-actin ?6high CD34high cells from postnatal d49 mouse back skins for RNA extraction and hybridization on Affymetrix microarrays. By comparing the transcriptional profile of FACS-isolated GFP ?6high CD34high expressing cells with FACS-isolated total GFP cells, we determined the genes differentially regulated in basal bulge stem cells in telogen stage comparing to total epidermis
2012-10-19 | E-GEOD-41704 | biostudies-arrayexpress
Project description:Epidermal appendages play a key role in thermoregulation, which is crucial in maintaining the equilibrium of body temperature. The normality of eccrine sweat glands (SwGs) and hair follicles (HFs) assures homeostatic thermoregulation. However, the fate commitments of SwGs and HFs development and their key regulators are still poorly understood. To elucidate the regulatory networks and the major regulators in SwGs and HFs development, we generated a transcriptomic atlas within three tissues (dorsal back skin, front and hinder ventral foot skin) across four early development stages (embryonic (E) days E15.5 and E17.5, postnatal (P) days P1 and P3) of Mus musculus. We then applied a second-order polynomial model to fit time-course expression of genes and used likelihood ratio test (LRT) to identify temporal dynamic expressed genes. We further constructed temporal regulatory networks to investigate the master regulators during different developmental stages. We found the distinct temporally developmental patterns between dorsal back and ventral foot skin. Eight temporally dynamic expression modes were inferred, which are comprised of over 90% of the dynamic genes. Shh, Eda, Bmp2 and other previously identified factors were noted in our dominant dynamic patterns as well and temporally dynamic expressed in dorsal back and ventral foot. Combined with differentially expressed genes analysis, we highlighted 501 genes that were common in front and hinder foot, albeit uniquely enrich or depleted in dorsal back. Moreover, the integration of temporal expression models and genes regulatory networks accentuated master transcription factors functioning at specific stages in different tissues. Our findings demonstrated the temporally dynamic determinants during SwGs and HFs fate commitments and can contribute to restoring impaired SwGs and HFs clinically after hypohidrosis or burn injury.
2024-08-31 | GSE237102 | GEO
Project description:Dkk4 transgenic mice in wild-type background showed abnormal hair subtype formation. Gene expression profiling showed significant down regulation of hair follicle-related keratin genes and hair keratin-associated protein genes at E18.5 and P1. Keywords: development or differentiation design,genetic modification design To define target genes of Dkk4 during hair follicle development, we carried out microarray experiments with mouse back skin samples that from 4 developmental time points including E14.5, E16.5, E18.5 and P1 of wild-type and wild-type background Dkk4 transgenic mice.
2010-09-03 | E-GEOD-19309 | biostudies-arrayexpress