Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of N2 and HBR227 worms in L1 arrest (48 hours)

ABSTRACT: We report the gene expression in L1 arrest (48 hours) of wild-type sleeping worms (N2) and aptf-1(gk794) mutant sleepless worms (HBR227)

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE196158 | GEO | 2022/02/28

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA-seq of N2, HBR2340 and PHX2193 worms in L1 arrest (48 hours)

Project description:We report the gene expression in L1 arrest (48 hours) of wild-type sleeping worms (N2) and RIS-manipulated worms (HBR2340 and PHX2193)

2022-02-28 | GSE196380 | GEO

RNA-seq of N2 and HBR227 worms in L1 arrest (48 hours)

Project description:RNA-seq of N2 and HBR227 worms in L1 arrest (48 hours)

| PRJNA803492 | ENA

RNA-seq of N2, HBR2340 and PHX2193 worms in L1 arrest (48 hours)

Project description:RNA-seq of N2, HBR2340 and PHX2193 worms in L1 arrest (48 hours)

| PRJNA804549 | ENA

Transcriptional profiling of C. elegans aptf-1(gk7954) mutant in L4 sleep stage.

Project description:To investigate which genes are up- or down-regulated during L4 sleep in C. elegans mutant aptf-1(gk794) we performed whole genome microarray expression profiling using the C. elegans (V2) Gene Expression Microarray, 4x44K from Agilent Technologies. Approximately 200 sleeping L4 worms were used per sample. As a control we have used N2 C. elegans wild type strain. Mutant condition (aptf-1(gk794)) and control (N2) were done in four replicates.

2015-09-22 | E-GEOD-73282 | biostudies-arrayexpress

RNA-seq analysis of L1 arrest linc-133(aqz 3) and WT worms

Project description:We used RNA-seq analysis to identify changes in gene expression occurring in linc-133 (aqz 3) mutants comparing with that in wild type (N2) worms during L1 arrest stage. Basing on these sequencing data, genes which were up-regulated or down-regulated by at least 2 times( |log2(FoldChange)| ≥ 1 and padj ≤ 0.05) subsequent to WT worms were analyzed. These differentially expressed genes were analyzed for pathway and gene ontology (GO) enrichment, which indicated that the genes influenced by aqz 3 mutation of linc-133 gene were enriched in several significant signaling pathways, including longevity, protein degradation in endoplasmic reticulum pathway, and fat metabolism pathways.

2023-01-09 | GSE222091 | GEO

Adult Caenorhabditis elegans: wild-type (N2) and mir-243 mutant worms

Project description:Transcriptional profiling of N2 vs. mir-243 worms, aiming to identify direct and indirect targets of the microRNA.

2011-09-16 | GSE20558 | GEO

Adult Caenorhabditis elegans: wild-type (N2) and mir-243 mutant worms

Project description:Transcriptional profiling of N2 vs. mir-243 worms, aiming to identify direct and indirect targets of the microRNA. N2 and mir-243 young-adult worms grown at 20C were analyzed. Three biological replicates with dye-swaps were performed.

2011-09-15 | E-GEOD-20558 | biostudies-arrayexpress

Transcriptome of wild type N2 worms at embronic stage and starved L1 stage

Project description:Transcription factors are key components of regulatory networks that control development, as well as the response to environmental stimuli. We have established an experimental pipeline in C. elegans that permits global identification of the binding sites for transcription factors using chromatin immunoprecipitation and deep sequencing. We describe and validate this strategy, and apply it to the transcription factor PHA-4, which plays critical roles in organ development and starvation stress response. We defined binding sites for PHA-4 during formation of the embryonic pharynx, and in starved larvae. PHA-4 binds to thousands of locations throughout the genome. These sites shift dramatically between embryos and larvae, from developmentally regulated genes to genes involved in metabolism. We acquired transcriptome of wild type N2 worms at embyonic and starved L1 stages using RNA-seq method in comparing to the ChIP-seq experiment to study transcription factor binding. The wild type worms were cultured the same as the transgenic worms for transcription factor binding experiment. Keywords: Transcriptome Analysis For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Two development stages embryo and starved larvae each contains 3 technical replicates are reported

2009-06-11 | E-GEOD-16552 | biostudies-arrayexpress

Transcriptome of wild type N2 worms at embronic stage and starved L1 stage

2009-06-11 | GSE16552 | GEO

N2 worms treated with Resveratrol

Project description:Eggs were isolated by hypochlorite treatment and allowed to hatch in the absence of food as L1 stage arrested worms in S-buffer. Worms were then transferred to large plates containing NGM alone or NGM+ resveratrol and allowed to grow till young adult stage (ca 40 hours). Worms were harvested and total RNA extracted using Trizol reagent. Poly-A+ RNA was isolated using Qiagen midi-prep kit and used for microarray hybridization. A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. Compound Based Treatment: Resveratrol Computed

2006-01-24 | E-GEOD-4094 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data