Global cardiac gene expression in left ventricles from 20 day-old and 60 day-old Efnb1 CMspe KO and WT male mice
Ontology highlight
ABSTRACT: To identify a physiologic postanal transcriptomic program between postnatal day 20 and 60, we first analyzed the gene expression profile in 60 day-old ( young adult) wild-type mice (WT) and compared it to 20 day-old WT mice To identify the gene expression between postnatal day 20 and 60 under the strict dependence of cardiac ephrin-B1, we compared gene expression in 20 day-old and 60 day-old Efnb1 CMspe KO (a-MHC-Cre-/+ Efnb1 flox/flox or) to 20 day-old and 60 day-old WT mice
Project description:9-day-old Sertoli cells have a stronger proliferative activity compared with 21-day-old Sertoli cells. We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated and down-regulated genes during this process. Rat Sertoli cell were selected at postnatal day 9 and postnatal day 21 for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain the differentially expressed genes that were involved in cell proliferation, cell cycle, and cell death which were associated with the function of Sertoli cell.
Project description:RNA immunoprecipitation assay followed by Next-Generation Sequencing (RIPseq) to identify RNAs bound by DDX20 in testis of postnatal 7-day-old C57BL/6 mice.
Project description:9-day-old Sertoli cells have a stronger proliferative activity compared with 21-day-old Sertoli cells. We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated and down-regulated genes during this process.
Project description:14-day-old seedling of WT, val1, val2, and val1val2 were performed RNA-seq to analyzed their differential expression genes in Arabidopsis.
Project description:To investigate the function of Znhit1 in mouse hearts, we deleted Znhit1 using conditional Znhit1-knockout mice (Znhit1 flox/flox; Myh6-Cre; Znhit1 cKO). Through RNA-Seq, we tested the genome-wide transcriptional changes at postnatal day 17 and 25.
Project description:To compare four- and seven-day-old Arabidopsis thaliana plasmodesmata proteome, the identification and the relative amount of proteins in plasmodesmata, cell wall and total cell extracts were performed using fractions purified from four- and seven-day-old liquid culture cells of A. thaliana. The relative amount for each protein was determined with a label-free quantification method. Four biological replicates of each fraction were used for quantification.
Project description:Purpose: To understand MAC3BQ77A/D81A induced abnormal immunity, we performed the whole genome transcriptome analysis on 7-day-old seedlings of WT, 35S:MAC3BQ77A/D81A-GFP. Conclusions: MAC3BQ77A/D81A positively regulates immune response.
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived retinal transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis Methods: RNA from 14-day-old seedlings of wild-type and pkl was isolated using the RNAprep Pure Plant Kit as described above. RNAs from three biological replicates was sequenced separately at Novogene, using Illumina Hiseq X-Ten (Sequencing method: Hiseq-PE150). Results: Reads were mapped to the TAIR10 Arabidopsis genome using TopHat (Galaxy V2.1.1 in usegalaxy.org) with default settings, except that a minimum intron length of 20 bp and a maximum intron length of 5,000 bp were required (Paired-end). Then, mapped reads were assembled according to TAIR10 version of genome annotation using cufflinks (version 2.1.1) with default settings. To analyze differential expression, the assembled transcripts from three independent biological replicates in Col and other mutants were included and compared using Cuffdiff (version 2.1.1) with default settings. 14-day-old seedling of WT and pkl were performed RNA-seq to analyzed their differential expression genes in Arabidopsis.