Project description:Activation of the Heat Shock Factor 1 (HSF1) in spermatogenic cells leads to apoptosis and infertility of males. To elucidate a mechanisms of apoptosis induced by HSF1 we generated transgenic mice expressing mutated, constitutively active human HSF1 (with a deletion of amino acids 221–315) specifically in spermatocytes (under control of the rat Hspa2 promoter). We carried out genome-wide transcriptional analyses in testes of control, wild-type, and transgenic males. Genes that changed the expression due to activation of HSF1 have been identified.
Project description:Expression changes in testes of 15-20 week old mice after knockout of Phf13 were analyzed using Affymetrix mouse genome 430 2.0 expression microarrays. Transcripts on the X- and Y-chromosome were significantly upregulated.
Project description:We performed RNA-sequencing of testes derived from Atr+/+ or Atr+/KD 7 weeks old mice. Since Atr+/KD male mice are sterile and present severe spermatogenesis defects, we aimed at analyzing the possible changes in gene expression given by the ATR kinase-dead (KD) mutation
Project description:9-day-old Sertoli cells have a stronger proliferative activity compared with 21-day-old Sertoli cells. We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated and down-regulated genes during this process.
Project description:Testes and testes terminal epithelium/seminal vesicles were dissected and separated from 4-5 day old Oregon-R-modENCODE Drosophila melanogaster males. RNA-sequencing was then performed on these two distinct biological structures.
Project description:Transcriptome-wide analysis of gene expression using detached first-pair rosette leaves before culture (time 0) and 1 day after culture (DAC) from 9-day-old, 12-day-old and 15-day-old Col-0 seedlings