Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RT-qPCR of metabolite and ICB exposed organoids

ABSTRACT: Organoids containing 4T1 TNBC and matched splenocytes were exposed to the metabolites hippuric acid or pyocyanin and/or a-CTLA-4 and a-PD-1 for 5 days run on RT² Profiler™ PCR Array Mouse Cancer Inflammation & Immunity Crosstalk [PMAM-181Z]

ORGANISM(S): Mus musculus

PROVIDER: GSE196268 | GEO | 2022/02/08

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RT-qPCR of metabolite and ICB exposed organoids

Project description:RT-qPCR of metabolite and ICB exposed organoids

| PRJNA804125 | ENA

RT² Profiler™ PCR Array Human Senesence

Project description:RT² Profiler™ PCR Array Human Senesence

| PRJNA594938 | ENA

RT² Profiler™ PCR Array Human Aging

Project description:RT² Profiler™ PCR Array Human Aging

| PRJNA594942 | ENA

Genetic profiling of breast cancer stem cell spheres exposed to immune surveillence

Project description:mouse 4T1 breast cancer stem cell spheres were co-culutred with in vivo tumor antigen primed splenocytes, with in vivo tumor antigen primed splenocytes plus ex vivo reinforced activation via anti-CD3/CD28 beads or without co-culturing with splenocytes. Stem cell spheres were then collected and sunjected for gene expression analyses using RNA sequencing.

2022-04-06 | GSE199983 | GEO

BCG stimulated C57BL/6-derived BMDM

Project description:BCG stimulated C57BL/6-derived BMDM, using PAMM-150Z RT2 Profiler™ PCR Array for Mouse Cytokines and Chemokines

2019-11-02 | GSE139864 | GEO

scRNAseq of CD45+ cells within PDAC tumors treateted with CD40/ICB

Project description:Goal: determine how transcriptome of immune cells within the tumor microenvironment changes as a function of CD40 agonist and/or immune checkpoint blockade (anti-PD-1 AND anti-CTLA-4; "ICB") therapy

2020-05-10 | GSE150176 | GEO

Real-time quantitative PCR analysis of WT and NOD1 knockout zebrafish (mTor)

Project description:Zebrafish larvae from WT and NOD1-1IS-/- collected at 10 dpf were used for Zebrafish mTOR Signaling RT² Profiler™ PCR Array.

2017-06-23 | GSE80621 | GEO

TLR signaling in C57BL/6J TLR2,4,7-tolerant splenocytes over PBS-treated counterpart.

Project description:Murine splenocytes were isolated from the spleens of C57BL/6J mice and were pretreated in vitro for three days in the presence of Pam3CSK4 (1 μg/ml), high pure LPS from E.coli O111:B4 (100 ng/ml) and R848 (5 μg/ml). PBS-treated splenocytes served as negative control. We used Qiagen Toll-like Receptor RT2 Profiler PCR Array kit to quantitate gene expression profiling of the TLR signaling pathway.

2017-04-21 | GSE80572 | GEO

Real-time quantitative PCR analysis of Hepatic cell lines infected with HCV

Project description:huh7.5 hepatic cell lines were infected with HCV. RNA and Protein were extract after 72 hours and The Human Adipogenesis RT² Profiler™ PCR Array was used to compare the gene expression in the two group.

2014-03-07 | GSE55677 | GEO

High-throughput microfluidic single-cell RT-qPCR.

Project description:A long-sought milestone in microfluidics research has been the development of integrated technology for scalable analysis of transcription in single cells. Here we present a fully integrated microfluidic device capable of performing high-precision RT-qPCR measurements of gene expression from hundreds of single cells per run. Our device executes all steps of single-cell processing, including cell capture, cell lysis, reverse transcription, and quantitative PCR. In addition to higher throughput and reduced cost, we show that nanoliter volume processing reduced measurement noise, increased sensitivity, and provided single nucleotide specificity. We apply this technology to 3,300 single-cell measurements of (i) miRNA expression in K562 cells, (ii) coregulation of a miRNA and one of its target transcripts during differentiation in embryonic stem cells, and (iii) single nucleotide variant detection in primary lobular breast cancer cells. The core functionality established here provides the foundation from which a variety of on-chip single-cell transcription analyses will be developed.

| S-EPMC3161570 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data