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TLR signaling in C57BL/6J TLR2,4,7-tolerant splenocytes over PBS-treated counterpart.

ABSTRACT: Murine splenocytes were isolated from the spleens of C57BL/6J mice and were pretreated in vitro for three days in the presence of Pam3CSK4 (1 μg/ml), high pure LPS from E.coli O111:B4 (100 ng/ml) and R848 (5 μg/ml). PBS-treated splenocytes served as negative control. We used Qiagen Toll-like Receptor RT2 Profiler PCR Array kit to quantitate gene expression profiling of the TLR signaling pathway.

ORGANISM(S): Mus musculus

PROVIDER: GSE80572 | GEO | 2017/04/21

SECONDARY ACCESSION(S): PRJNA319334

REPOSITORIES: GEO

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