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Improved methods for deamination-based m6A detection


ABSTRACT: We present an improved version of DART-seq which utilizes a variant of the YTH domain engineered to achieve enhanced m6A recognition in APO1-YTH (D422N). In addition, we develop in vitro DART-seq and show that it performs similarly to cellular DART-seq and can map m6A in any sample of interest using nanogram amounts of total RNA.

ORGANISM(S): Homo sapiens

PROVIDER: GSE196416 | GEO | 2022/05/11

REPOSITORIES: GEO

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