Dataset Information

MS-based characterization of YTHDC1 interactome

ABSTRACT: Heat shock induces a cell response leading to profound changes in genome expression. Recently, N6-methyladenosine (m6A) RNA modification has been implicated in this response, but with limited information of its role in the heat-induced reprograming of gene expression. Most of m6A molecular and cellular functions rely on m6A readers and the best characterized m6A readers are members of the YTH-domain-containing protein family present from yeast to humans. To investigate the function of the nuclear m6A reader YTHDC1, we characterized its binding partners.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Fibroblast

SUBMITTER: Yohann Couté

LAB HEAD: Yohann Couté

PROVIDER: PXD037414 | Pride | 2023-01-11

REPOSITORIES: Pride

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Chromatin-associated YTHDC1 coordinates heat-induced reprogramming of gene expression.

Timcheva Kalina K Dufour Solenne S Touat-Todeschini Leila L Burnard Callum C Carpentier Marie-Christine MC Chuffart Florent F Merret Rémy R Helsmoortel Marion M Ferré Sabrina S Grézy Aude A Couté Yohann Y Rousseaux Sophie S Khochbin Saadi S Vourc'h Claire C Bousquet-Antonelli Cécile C Kiernan Rosemary R Seigneurin-Berny Daphné D Verdel André A

Cell reports 20221201 11

Heat stress (HS) induces a cellular response leading to profound changes in gene expression. Here, we show that human YTHDC1, a reader of N6-methyladenosine (m6A) RNA modification, mostly associates to the chromatin fraction and that HS induces a redistribution of YTHDC1 across the genome, including to heat-induced heat shock protein (HSP) genes. YTHDC1 binding to m6A-modified HSP transcripts co-transcriptionally promotes expression of HSPs. In parallel, hundreds ...[more]

PMID: 36516773

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