The effect of exogenous strigolactone on gene expression in Arabidopsis seedlings
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ABSTRACT:
ORGANISM(S): Arabidopsis thaliana
PROVIDER: GSE19661 | GEO | 2009/12/28
SECONDARY ACCESSION(S): PRJNA122563
REPOSITORIES: GEO
Similar Datasets
Project description:Strigolactones have been defined as a new group of phytohormones that regulate shoot branching. Tthe phenotypes of strigolacton-related rice (Oryza sativa) dwarf (d) mutants demonstrated that SLs inhibit mesocotyl elongation by controlling cell division. Moreover, the trans-zeatin, one of active cytokinins, content of mesocotyls was increased in the SL-deficient d10-1 and SL-insensitive d14-1 mutants. To examine if there are genes related to cytokinin-biosynthesis or -degradation among the strigolactone-responsive genes expressed in the mesocotyl, we carried out microarray analyses using d10-1, d14-1 and the wild-type grown under dark conditions, with or without pretreatment of a synthetic strigolactone analog, GR24.
2013-12-31 | GSE36271 | GEO
Project description:Strigolactones (SLs) are carotenoid-derived plant hormones that control shoot branching and communications between host plants and symbiotic fungi or root parasitic plants. Extensive studies have identified the key components participating in SL biosynthesis and signaling, whereas the catabolism or deactivation of endogenous SLs in planta remains largely unknown. Here we report that the Arabidopsis carboxylesterase 15 (AtCXE15) and its orthologues function as efficient hydrolases of SLs. We show that overexpression of AtCXE15 promotes shoot branching by dampening SL-inhibited axillary bud outgrowth. We further demonstrate that AtCXE15 could bind and efficiently hydrolyze SLs both in vitro and in planta. We also provide evidence that AtCXE15 is capable to catalyze hydrolysis of diverse SL analogues and that such CXE15-dependent catabolism of SLs is evolutionarily conserved in seed plants. These results disclose a catalytic mechanism underlying homeostatic regulation of SLs in plants, which also provides a rational approach to spatial-temporally manipulate the endogenous SLs and thus architecture of crops and ornamental plants.
2021-08-05 | GSE176007 | GEO
Project description:Strigolactones (SLs), a class of phytohormones, play a pivotal role in governing critical aspects of plant development, including shoot branching and root development. DWARF53 (D53) is a key repressor in the SL signaling. Nevertheless, its involvement and regulatory mechanisms in cotton fiber development—a representative single-cell model, has remained elusive. In this study, we unveil GhD53 represses the transcription of SL-inducible genes GhFAD3-2 and GhFAD3-4, which are involved in linolenic acid biosynthesis—a stimulant of cell elongation, thus inhibiting cotton fiber elongation. We demonstrate that GhD53's inhibitory influence on cotton fiber elongation is relieved in the presence of SLs, as GhD53 undergoes degradation. This alleviation of GhD53 repression triggers the activation of GhFAD3 expression, promoting fiber cell elongation. Remarkably, GhD53 serves as a dual-function transcription repressor, impeding SLs’ role in fiber elongation. Firstly, as a transcription factor, GhD53 directly binds to GhFAD3 promoters, suppressing their transcription. Secondly, GhD53 physically interacts with GhSLRF, a bHLH-type transcription factor, preventing it from binding to GhFAD3 promoters and repressing its transcription activation activity. GhD53 emerges as a pivotal component in the regulatory network governing SL signaling transduction. Overall, our findings provide novel insights into D53’s role in SL signaling and delineate a comprehensive framework for transmitting SL signals to modulate plant cell elongation behavior.
2024-09-01 | GSE245581 | GEO
Project description:Strigolactones have been defined as a new group of phytohormones that regulate shoot branching. The phenotypes of strigolacton-related rice (Oryza sativa) dwarf (d) mutants demonstrated that SLs inhibit mesocotyl elongation by controlling cell division. Moreover, the trans-zeatin, one of active cytokinins, content of mesocotyls was increased in the SL-deficient d10-1 and SL-insensitive d14-1 mutants. To examine if there are genes related to cytokinin-biosynthesis or -degradation among the strigolactone-responsive genes expressed in the mesocotyl, we carried out microarray analyses using d10-1, d14-1 and the wild-type grown under dark conditions, with or without pretreatment of a synthetic strigolactone analog, GR24. Gene expression profiles in 4-day-old mesocotyls of dwarf (d) mutants (d10-1 and d14-1) and wild-type germinated under dark conditions with or without treatment of 1 μM GR24 were analyzed. Three biological replicates were prepared for each conditions, and a total of eighteen samples were analyzed.
2013-12-31 | E-GEOD-36271 | biostudies-arrayexpress
Project description:Purpose: Strigolactones (SLs), a class of carotenoid-derived plant hormones, have fundamental effects on plant development. But only a few SL-responsive genes have been identified. The goal of this study is to identify SL early responsive genes in the model system Arabidopsis thaliana. Methods: The 10-day-old wild-type seedlings were treated with GR244DO or solvent for 2 h or 4 h. The mRNA profiles were generated by deep sequencing, in triplicate, using Illumina system Hiseq2000. The sequence reads were analyzed using STAR and R package DESeq. To limit interference of circadian clock and environmental factors, GR244DO treatment at 2 h was compared to solvent treatment control at 2 h as well as the start point of treatment, and genes upregulated at both conditions were considered as up-regulated SL responsive genes at 2 h. The same comparison criteria were applied to identify down-regulated responsive genes at 4 h. qRT-PCR validation was performed using CFX 96 real-time PCR detection system. Results: We identified 99 upregulated and 57 downregulated genes at 2 h treatment, as well as 147 upregulated and 150 downregulated genes at 4 h treatment in Arabidopsis thaliana using the cutoff of fold-change more than 1.5 and P-value less than 0.05.
2020-03-16 | GSE126331 | GEO
Project description:Identification of strigolactone-dependent genes in barley. Comparison of two organs (shoot and root) allowed to identify tissue specific genes remains under control of strigolactones in barley
2024-07-26 | E-MTAB-13641 | biostudies-arrayexpress
Project description:The aim of this study was to investigate the role of strigolactones (SLs) in controlling tillering and their involvement as a signal for nitrogen status. RNA-seq analysis was performed on 18-day-old hydroponically grown Tad17 triple knock-out TILLING mutant plants (SL-deficient mutant) and WT-segregant plants, 8 days after introducing them to nitrogen limiting conditions. The experiment consisted of two different treatments: High Nitrogen (10 mM N) and Low Nitrogen (0.1 mM N). Each treatment included six biological replicates. Total RNA was extracted from pooled samples of four basal node sections per biological replicate. In this study, the basal node was defined as the 0.5 cm section from the base of the main shoot, comprising the apical meristem, lateral buds, leaf meristems, etc.
2024-08-13 | E-MTAB-14014 | biostudies-arrayexpress
Project description:Strigolactones (SLs) are plant hormones that regulate diverse developmental processes and environmental responses in plants. It has been discovered that SLs play an important role in regulating plant immune resistance to pathogens, but there are currently no reports on their role in the interaction between Nicotiana benthamiana and Tobacco mosaic virus (TMV). In this study, the exogenous application of SLs weakened the resistance of N. benthamiana to TMV, promoting TMV infection, whereas the exogenous application of Tis108, an SL inhibitor, resulted in the opposite effect. Virus-induced gene silencing (VIGS) inhibition of two key SL synthesis enzyme genes, NtCCD7 and NtCCD8, enhanced the resistance of N. benthamiana to TMV. Additionally, we conducted a screening of N. benthamiana related to TMV infection. TMV-infected plants treated with SLs were compared to the control by using RNA-seq. KEGG enrichment analysis and weighted gene co-expression network analysis (WGCNA) of differentially expressed genes (DEGs) suggested that plant hormone signaling transduction may play a significant role in the SL-TMV-N. benthamiana interactions. This study reveals new functions of SLs in regulating plant immunity and provides a reference for controlling TMV diseases in production.
2024-08-16 | GSE272661 | GEO
Project description:Strigolactones (SL) are a new class of plant hormones with a broad range of roles in symbiosis, germination and developmental regulation. The well-characterized signaling pathway for SL involves the canonical signaling component MAX2. Here we identified a novel, MAX2-independent SL signaling mechanism, which initiates in mitochondria, signals to the nucleus and regulates root development. By a forward genetic approach, we identified the SL-insensitive pig1 mutant, which altered the kinetic properties of the mitochondrial pyruvate dehydrogenase complex. SL has an unknown target in mitochondria, where it uncouples respiration. This leads to metabolic aberrations, mitochondria-to-nucleus retrograde signaling upregulating transcription of mitochondrial dysfunction genes and downregulating auxin-regulated genes. SL-triggered mitochondrial uncoupling is communicated to the nucleus via cleavage and translocation of the ER-localized transcription factor ANAC017. These observations establish a novel mechanism for SL signaling emanating from mitochondria and regulating development.
2022-04-18 | GSE200634 | GEO
Project description:The gaseous phytohormone ethylene influences many aspects of plant life, including germination, fruit ripening, senescence, and stress responses. These diverse roles of ethylene occur in part through crosstalk with other phytohormones, which affects ethylene biosynthesis and signaling pathways. We have recently shown that the phytohormones, including gibberellic acid, abscisic acid, auxin, methyl jasmonate, and salicylic acid, regulate the stability of ACC synthases (ACSs), the rate-limiting enzymes in ethylene biosynthesis. Here, we report that treatment of etiolated Arabidopsis seedlings with strigolactone (SL) increases ethylene biosynthesis. SL does not influence ACS stability or ACS gene expression, but it increases the transcript levels of a subset of ACC oxidase (ACO) genes, thereby enhancing ethylene biosynthesis. Taken together with the results of our previous study, these findings demonstrate that most phytohormones differentially regulate ethylene biosynthesis in dark-grown Arabidopsis seedlings by affecting ACS stability and/or the transcript levels of ethylene biosynthesis genes.
| S-EPMC7588227 | biostudies-literature