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Genome-wide identification of genes critical for in vivo fitness of porcine extraintestinal pathogenic Escherichia coli by using transposon-directed insertion site sequencing

ABSTRACT: This study was aimed to do genome-wide identification of fitness factors of ExPEC using an mouse infection model. A transposon (Tn) mutagenesis library (input) containing insertions of 72% of the total chromomose encoded genes was used to infect mouse. Bacteria were then recovered from the brain, lung, and spleen (output libraries) at 12 hours post infection. The genomic DNA was extracted from the input and the output libraries. The genomic fragment flanking the transposon was enriched by PCR which was then sequenced by Illumina sequencing. The insertion sites were then mapped to the genome of ExPEC PCN033 strain to identify differentially depleted genes which are genes potentially involved in fitness during infection.

ORGANISM(S): Escherichia coli PCN033

PROVIDER: GSE197084 | GEO | 2022/04/30

REPOSITORIES: GEO

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