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5' UTR-dependent m6A methylation regulates aging and circadian rhythms in Drosophila [RIP-seq whole]

ABSTRACT: To evoke further attention to the potential hazard of increasingly accumulative blue light exposure, we construct a series of in vivo Drosophila models employed for multi-omics analyses. This project includes the identification results of untargeted metabolome quantification by LC-MS/MS and the Input and m6A IP data of MeRIP-seq of w1118 male adult whole flies.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE197395 | GEO | 2023/11/08

REPOSITORIES: GEO

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5' UTR-dependent m6A methylation regulates aging and circadian rhythms in Drosophila [RNA-seq head]

Project description:To evoke further attention to the potential hazard of increasingly accumulative blue light exposure, we construct a series of in vivo Drosophila models employed for multi-omics analyses. This project includes the RNA-seq data of w1118 male adult heads.

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5' UTR-dependent m6A methylation regulates aging and circadian rhythms in Drosophila [RNA-seq whole]

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<i>Period2</i> 3'-UTR and microRNA-24 regulate circadian rhythms by repressing PERIOD2 protein accumulation.

Project description:We previously created two PER2::LUCIFERASE (PER2::LUC) circadian reporter knockin mice that differ only in the Per2 3'-UTR region: Per2::Luc, which retains the endogenous Per2 3'-UTR and Per2::LucSV, where the endogenous Per2 3'-UTR was replaced by an SV40 late poly(A) signal. To delineate the in vivo functions of Per2 3'-UTR, we analyzed circadian rhythms of Per2::LucSV mice. Interestingly, Per2::LucSV mice displayed more than threefold stronger amplitude in bioluminescence rhythms than Per2::Luc mice, and also exhibited lengthened free-running periods (?24.0 h), greater phase delays following light pulse, and enhanced temperature compensation relative to Per2::Luc Analysis of the Per2 3'-UTR sequence revealed that miR-24, and to a lesser degree miR-30, suppressed PER2 protein translation, and the reversal of this inhibition in Per2::LucSV augmented PER2::LUC protein level and oscillatory amplitude. Interestingly, Bmal1 mRNA and protein oscillatory amplitude as well as CRY1 protein oscillation were increased in Per2::LucSV mice, suggesting rhythmic overexpression of PER2 enhances expression of Per2 and other core clock genes. Together, these studies provide important mechanistic insights into the regulatory roles of Per2 3'-UTR, miR-24, and PER2 in Per2 expression and core clock function.

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5' UTR-dependent m6A methylation regulates aging and circadian rhythms in Drosophila

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-11-08 | GSE197397 | GEO

MeRIP, RIP and RNA Sequencing of the H1299 stable cell lines which the endogenous YTHDF2 was silenced by a short hairpin RNA targeting YTHDF2 3’UTR (shYTHDF2) and then re-expressed Flag-tagged YTHDF2-WT orYTHDF2-K571R

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Microarray experiment to identify gene expression changes in response to Phenobarbital treatment and CncC overexpression in Drosophila.

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