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ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection I

ABSTRACT: In this study, we define ZBTB7A as a driver of cellular survival during HCoV-229E infection.

ORGANISM(S): Homo sapiens

PROVIDER: GSE197644 | GEO | 2022/10/05

REPOSITORIES: GEO

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ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection II

Project description:In this study, we define ZBTB7A as a driver of cellular survival during HCoV-229E infection.

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ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection

Project description:ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection

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ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection II

Project description:ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection II

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ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection I

Project description:ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection I

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ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection.

Project description:The cellular fate after infection with human coronaviruses (HCoVs) is typically death. Previous data suggest, however, that the transcriptional state of an individual cell may sometimes allow additional outcomes of infection. Here, to probe the range of interactions a permissive cell type can have with a HCoV, we perform a CRISPR activation screen with HCoV-229E. The screen identified the transcription factor ZBTB7A, which strongly promotes cell survival after infection. Rather than suppressing viral infection, ZBTB7A upregulation allows the virus to induce a persistent infection and homeostatic state with the cell. We also find that control of oxidative stress is a primary driver of cellular survival during HCoV-229E infection. These data illustrate that, in addition to the nature of the infecting virus and the type of cell that it encounters, the cellular gene expression profile prior to infection can affect the eventual fate.

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Transcriptomic profiling of human corona virus (HCoV)-229E -infected human cells

Project description:Human coronaviruses (HCoVs) cause mild to severe respiratory infection. Most of the common cold illnesses are caused by one of four HCoVs, namely HCoV-229E, HCoV-NL63, HCoV-HKU1 and HCoV-OC43. Several studies have applied global transcriptomic methods to understand host responses to HCoV infection, with most studies focusing on the pandemic severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome CoV (MERS-CoV) and the newly emerging SARS-CoV-2. In this study, Next Generation Sequencing was used to gain new insights into cellular transcriptomic changes elicited by alphacoronavirus HCoV-229E. HCoV-229E-infected MRC5 cells showed marked downregulation of superpathway of cholesterol biosynthesis and eIF2 signaling pathways. Moreover, upregulation of cyclins, cell cycle control of chromosomal replication, and role of BRCA1 in DNA damage response, alongside downregulation of the cell cycle G1/S checkpoint, suggest that HCoV-229E favors S phase for viral infection. Intriguingly, more than 80% of key factors of cell innate immunity, interferon-stimulated genes (ISGs) and other transcripts of early antiviral response genes were downregulated early in HCoV-229E infection. This study will enhance our understanding of commonly circulating HCoVs and hopefully provide critical information about still-emerging coronaviruses.

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Genome-scale CRISPR knockout screen for Dengue, Japanese Encephalitis, and Human Coronavirus 229E viruses

Project description:Unbiased forward genetic screens to identify host factors for DENV1 and JEV in 293FT cells. Unbiased forward genetic screens to identify host factors for HCoV-229E in Huh7.5.1 cells.

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ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-10-05 | GSE197883 | GEO

HCoV-229E infection of the human HuH7 cell line [Experiment A]

Project description:The aim of the experiment is to identify changes in host gene expression upon infection of HuH7 human hepatoma cells with Human corona virus-229E (HCoV-229E) compared to mock-infected cells. All experiments were performed at 33°C which is required for sufficient viral entry/replication.

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p97/VCP as a therapy target for developing drugs against human coronavirus

Project description:Understanding how human coronavirus dysregulate host proteome during infection in human cells will identify general pathways that are common to coronavirus infection. NMS-873 is an allosteric p97/VCP ATPase inhibitor and was show to have antiviral effect in multiple viruses including SARS-CoV2. We first demonstrated that genetic knock down of p97 reduced HCoV-229E, HCoV-OC43 infection and secretion. To investigate how p97/VCP assists virus infection, we used unbiased quantitative proteomics to compare dysregulated proteomes caused by HCoV-229E, HCoV-OC43 and SARS-CoV2 infection. We then compared dysregulated proteomes after HCoV-229E and HCoV-OC43 infection with and without p97 knockdown. Moreover, we elucidated the impact of p97 on different stages of viral life cycle using two potent p97 inhibitors, CB-5083 and NMS-873 and demonstrate their can block HCoV replication. Together, our data provide insights to repurpose potential cancer drugs that target the essential host protein p97/VCP.

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