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ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection I

ABSTRACT: ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection I

PROVIDER: PRJNA811447 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18182068_1.fastq.gz	Fastqsanger.gz
	SRR18182068_2.fastq.gz	Fastqsanger.gz
	SRR18182069_1.fastq.gz	Fastqsanger.gz
	SRR18182069_2.fastq.gz	Fastqsanger.gz
	SRR18182070_1.fastq.gz	Fastqsanger.gz

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ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection II

Project description:In this study, we define ZBTB7A as a driver of cellular survival during HCoV-229E infection.

2022-10-05 | GSE197882 | GEO

ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection I

Project description:In this study, we define ZBTB7A as a driver of cellular survival during HCoV-229E infection.

2022-10-05 | GSE197644 | GEO

ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection

Project description:ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection

| PRJNA812582 | ENA

ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection II

Project description:ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection II

| PRJNA812586 | ENA

ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection.

Project description:The cellular fate after infection with human coronaviruses (HCoVs) is typically death. Previous data suggest, however, that the transcriptional state of an individual cell may sometimes allow additional outcomes of infection. Here, to probe the range of interactions a permissive cell type can have with a HCoV, we perform a CRISPR activation screen with HCoV-229E. The screen identified the transcription factor ZBTB7A, which strongly promotes cell survival after infection. Rather than suppressing viral infection, ZBTB7A upregulation allows the virus to induce a persistent infection and homeostatic state with the cell. We also find that control of oxidative stress is a primary driver of cellular survival during HCoV-229E infection. These data illustrate that, in addition to the nature of the infecting virus and the type of cell that it encounters, the cellular gene expression profile prior to infection can affect the eventual fate.

| S-EPMC9533670 | biostudies-literature

ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-10-05 | GSE197883 | GEO

Transcriptomic profiling of human corona virus (HCoV)-229E -infected human cells

Project description:Human coronaviruses (HCoVs) cause mild to severe respiratory infection. Most of the common cold illnesses are caused by one of four HCoVs, namely HCoV-229E, HCoV-NL63, HCoV-HKU1 and HCoV-OC43. Several studies have applied global transcriptomic methods to understand host responses to HCoV infection, with most studies focusing on the pandemic severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome CoV (MERS-CoV) and the newly emerging SARS-CoV-2. In this study, Next Generation Sequencing was used to gain new insights into cellular transcriptomic changes elicited by alphacoronavirus HCoV-229E. HCoV-229E-infected MRC5 cells showed marked downregulation of superpathway of cholesterol biosynthesis and eIF2 signaling pathways. Moreover, upregulation of cyclins, cell cycle control of chromosomal replication, and role of BRCA1 in DNA damage response, alongside downregulation of the cell cycle G1/S checkpoint, suggest that HCoV-229E favors S phase for viral infection. Intriguingly, more than 80% of key factors of cell innate immunity, interferon-stimulated genes (ISGs) and other transcripts of early antiviral response genes were downregulated early in HCoV-229E infection. This study will enhance our understanding of commonly circulating HCoVs and hopefully provide critical information about still-emerging coronaviruses.

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Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors

Project description:The ongoing SARS-CoV-2 pandemic has devastated the global economy and claimed more than one million lives, presenting an urgent global health crisis. To identify host factors required for infection by SARS-CoV-2 and seasonal coronaviruses, we designed a focused high-coverage CRISPR-Cas9 library targeting 332 members of a recently published SARS-CoV-2 protein interactome. We leveraged the compact nature of this library to systematically screen SARS-CoV-2 at two physiologically relevant temperatures (33 ºC and 37 ºC) along with three related coronaviruses (HCoV-229E, HCoV-NL63, and HCoV-OC43), allowing us to probe this interactome at a much higher resolution relative to genome scale studies. This approach yielded several new insights, including unexpected virus-specific differences in Rab GTPase requirements and GPI anchor biosynthesis, as well as identification of multiple pan-coronavirus factors involved in cholesterol homeostasis. This coronavirus essentiality catalog could inform ongoing drug development efforts aimed at intercepting and treating COVID-19, and help prepare for future coronavirus outbreaks.

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Genome-scale CRISPR knockout screen for Dengue, Japanese Encephalitis, and Human Coronavirus 229E viruses

Project description:Unbiased forward genetic screens to identify host factors for DENV1 and JEV in 293FT cells. Unbiased forward genetic screens to identify host factors for HCoV-229E in Huh7.5.1 cells.

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HCoV-229E infection of the human HuH7 cell line [Experiment B]

Project description:The aim of the experiment is to identify changes in host gene expression upon infection of Huh7 hepatoma carcinoma cells with human coronavirus HCoV-229E as compared to uninfected cells and in comparison to cells stimulated by IL-1alpha (10ng/ml) for 1h. All experiments have been performed at 33°C which is required for sufficient viral entry/replication. Also included are two samples which have been treated with a heat inactivated coronavirus (56°C for 5min).

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