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Functional crosstalk between the cohesin loader and a chromatin remodeler

ABSTRACT: The Scc2-Scc4 complex interacts with a conserved RSC ATPase motor module. The cohesin loader enhances RSC remodeling activity in vitro and promoter nucleosome eviction in vivo.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE197657 | GEO | 2022/03/01

REPOSITORIES: GEO

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Functional crosstalk between the cohesin loader and chromatin remodelers

Project description:The cohesin complex has crucial roles in many structural and functional aspects of chromosomes including sister chromatid cohesion, genome organization, gene transcription and DNA repair. Cohesin recruitment onto chromosomes requires nucleosome free DNA and a specialized cohesin loader complex comprised of the Scc2 and Scc4 subunits. The cohesin loader, in addition to stimulating cohesin ATP hydrolysis and facilitating topological loading onto DNA, leads cohesin to chromatin receptors such as the RSC chromatin remodeling complex. Here, we explore the cohesin loader-RSC interaction and show that its Scc4 subunit contacts a conserved RSC ATPase motor module. The cohesin loader enhances RSC chromatin remodeling activity in vitro, as well as promoter nucleosome eviction in vivo. These findings provide insight into how the cohesin loader recognizes, as well as influences, the chromatin landscape, with implications for our understanding of human developmental disorders including Cornelia de Lange and Coffin-Siris syndromes.

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Project description:The Scc2/Scc4 complex binds to broad nucleosome-free regions in the promoters of highly expressed genes. The cohesin loader is recruited to these sites by the RSC chromatin remodeling complex Total RNA was isolated from wt, scc2-4 and sth1-3 cells at 25Â°C and 37Â°C. dscDNA was obtained using the IVT system (Invitrogen) and hybridized to Affymetrix Yeast Genome 2.0 Arrays to analyze the expression profile of wt, scc2-4 and sth1-3 cells.

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