Genomics

Dataset Information

0

Schlafen 5 Suppresses Human Immunodeficiency Virus Type 1 Transcription by Commandeering Cellular Epigenetic Machinery


ABSTRACT: Schlafen-5 (SLFN5) is an interferon-induced protein of the Schlafen family which are involved in immune responses and oncogenesis. To date, little is known regarding its anti-HIV-1 function. Here, the authors report that overexpression of SLFN5 inhibits HIV-1 replication and reduces viral mRNA levels, whereas depletion of endogenous SLFN5 promotes HIV-1 replication. Moreover, they show that SLFN5 markedly decreases the transcriptional activity of HIV-1 long terminal repeat (LTR) via binding to two sequences in the U5-R region, which consequently represses the recruitment of RNA polymerase Ⅱ to the transcription initiation site. Mutagenesis studies show the importance of nuclear localization and the N-terminal 1-570 amino acids fragment in the inhibition of HIV-1. Further mechanistic studies demonstrate that SLFN5 interacts with components of the PRC2 complex, G9a and Histone H3, thereby promoting H3K27me2 and H3K27me3 modification leading to silencing HIV-1 transcription. In concert with this, they find that SLFN5 blocks the activation of latent HIV-1. Altogether, their findings demonstrate that SLFN5 is a transcriptional repressor of HIV-1 through epigenetic modulation and a potential determinant of HIV-1 latency.

ORGANISM(S): Homo sapiens

PROVIDER: GSE197700 | GEO | 2022/05/24

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2020-12-21 | GSE140600 | GEO
2023-09-25 | GSE236931 | GEO
2023-09-25 | GSE236656 | GEO
2023-10-25 | GSE246082 | GEO
| PRJNA287455 | ENA
2016-08-09 | E-GEOD-84285 | biostudies-arrayexpress
2012-07-31 | E-GEOD-35864 | biostudies-arrayexpress
2018-06-06 | MTBLS449 | MetaboLights
2024-11-05 | GSE281025 | GEO
2021-08-31 | GSE157127 | GEO