Bacterial diacetyl suppresses abiotic stress-induced senescence in Arabidopsis
Ontology highlight
ABSTRACT: Plant premature senescence is a major reason for agricultural losses caused by abiotic stress. Tools for suppressing stress-induced plant senescence are important but rare. Herein we report that diacetyl, a natural compound emitted by a plant-beneficial bacterium, suppresses ABA-mediated foliar senescence in Arabidopsis thaliana under various abiotic stress conditions. Our results established diacetyl as an effective protector of stress-induced plant senescence and disclosed a molecular mechanism for bacteria-enhanced plant stress-resistance.
Project description:Here, we investigated the function of the plant-specific SR protein RS33 in pre-mRNA splicing regulation and abiotic stress responses in rice. The loss-of-function mutant, rs33, showed increased sensitivity to salt and low-temperature stress. Genome-wide analyses of gene expression and splicing in seedlings subjected to these stresses identified multiple splice isoforms from stress-responsive genes dependent on RS33. The number of RS33-regulated genes is much higher under low-temperature stress as compared to salt stress. Our results suggest that this plant-specific splicing factor plays crucial and distinct roles during plant adaptation to abiotic stresses.
Project description:Plant cyclophilins are widely involved in a variety of abiotic stress regulation processes. Thirty cyclophilin members were identified from apple and their patterns of response to abiotic stress were examined. Apple seedlings with consistent growth were selected, cultivated under drought stress conditions simulated by PEG6000, sampled according to different time periods, and quickly frozen in liquid nitrogen.Total RNAs of apple plantlets were isolated using an RNA extraction kit (Tiangen, Beijing, China).
Project description:Recent studies have shown that several plant species require microbial associations for stress tolerance and survival. In this work, we show that the desert endophytic bacterium Enterobacter sp. SA187 enhances yield and biomass of alfalfa in field trials, revealing a high potential for improving desert agriculture. To understand the underlying molecular mechanisms, we studied SA187 interaction with Arabidopsis thaliana. SA187 colonized surface and inner tissues of Arabidopsis roots and shoots and conferred tolerance to salt and osmotic stresses. Transcriptome, genetic and pharmacological studies revealed that the ethylene signaling pathway plays a key role in mediating SA187-triggered abiotic stress tolerance to plants. While plant ethylene production is not required, our data suggest that SA187 induces abiotic stress tolerance by bacterial production of 2-keto-4-methylthiobutyric acid (KMBA), known be converted into ethylene in planta. These results reveal a part of the complex molecular communication process during beneficial plant-microbe interactions and unravel an important role of ethylene in protecting plants under abiotic stress conditions.
Project description:The ultimate aim is see if rhizosphere microbiota are influenced by changes in root exudate composition resulting from abiotic stress. The abiotic variables we are focusing on at this stage are salinity, temperature and pH. This can be divided into two questions: (a) how do plant exudates change in response to abiotic stress, and (b) how do these changes influence bacteria. In order to test this we will produce plant exudates under controlled stressed conditions, measure their composition and measure bacterial growth in these exudates. Data has also been produced from synthetic community experiments comparing the community composition under a variety of controlled stress conditions (temperature, salinity, pH, and phosphate).
The work (proposal:https://doi.org/10.46936/10.25585/60000944) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.
Project description:The ultimate aim is see if rhizosphere microbiota are influenced by changes in root exudate composition resulting from abiotic stress. The abiotic variables we are focusing on at this stage are salinity, temperature and pH. This can be divided into two questions: (a) how do plant exudates change in response to abiotic stress, and (b) how do these changes influence bacteria. In order to test this we will produce plant exudates under controlled stressed conditions, measure their composition and measure bacterial growth in these exudates. Data has also been produced from synthetic community experiments comparing the community composition under a variety of controlled stress conditions (temperature, salinity, pH, and phosphate).
The work (proposal:https://doi.org/10.46936/10.25585/60000944) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.
Project description:We investigated the function of the plant-specific SR protein SC106 in pre-mRNA splicing regulation and abiotic stress responses in rice. The loss-of-function mutant, SC106, showed increased sensitivity to salt. We performed genome-wide analyses of gene expression and splicing in seedlings subjected to salt stress and identified multiple splice isoforms from stress-responsive genes dependent on SC106.
Project description:To assess the reproducibility of the microarray analysis, we repeated the experiment three times with independently prepared total RNA. Keywords = abiotic stress Keywords: repeat sample
Project description:Soil microbiota can confer fitness advantages to plants and increase crop resilience to drought and other abiotic stressors. However, there is little evidence on the mechanisms correlating a microbial trait with plant abiotic stress tolerance. Here, we report that a class of Streptomyces effectively alleviates the drought and salinity stress by producing new spiroketal polyketide pteridic acid H (1) and its isomer F (2). The bifunctional pteridic acid biosynthetic gene cluster (pta), which is also responsible for the biosynthesis of the known antimicrobial elaiophylin, was confirmed by bioinformatic analysis and in vivo CRISPR base editing. Pteridic acids H and F exhibited profound effects in promoting root growth in Arabidopsis at a concentration of 0.5 ng mL-1 (1.3 nM) under abiotic stress, indicating they are a new class of plant stress regulators. Phylogenetic and geographical distribution analysis revealed that the pta was mainly disseminated by vertical transmission and occasional horizontal gene transfer and is widely distributed in numerous Streptomyces in different environments. This discovery provides a new perspective for understanding plant-Streptomyces interactions and provides a novel, promising approach for utilising beneficial Streptomyces and their secondary metabolites in agriculture to mitigate the detrimental effects of climate change.
Project description:Background: Plant disease is a major challenge to agriculture worldwide, and it is often exacerbated by abiotic environmental factors. During some plant-pathogen interactions, heat stress increases host susceptibility, a tendency which could spell disaster in light of the global warming trends associated with climate change. Despite the importance of this phenomenon, little is known about the molecular mechanisms that cause it. To better understand host plant responses during simultaneous heat and pathogen stress, we conducted a transcriptomics experiment for rice plants infected with Xanthomonas oryzae (Xo), an economically important bacterial pathogen of rice, during high temperature stress. Results: Using RNA-Seq technology, 8,499 differentially expressed genes were identified as temperature responsive in one rice cultivar, IRBB61, experiencing susceptible and resistant interactions with Xo across three time points. Many genes with gene ontology terms associated with stress response were identified. Notably, genes in the plant hormone abscisic acid (ABA) biosynthesis and response pathways were identified as upregulated by high temperature in both mock-treated plants and plants in the susceptible interaction and suppressed by high temperature in plants in the resistant interaction. A DNA sequence motif similar to known ABA-responsive cis-regulatory elements was identified in the promoter region upstream of genes upregulated in susceptible but downregulated in resistant interactions. Conclusions: The results of our study suggest that the plant hormone ABA is an important node for cross-talk between plant transcriptional response pathways to high temperature stress and pathogen attack. Genes in this pathway represent an important focus for future study to determine how plants evolved to deal with simultaneous abiotic and biotic stresses.