Genomics

Dataset Information

0

A de novo transcription-dependent TAD boundary underpins critical multiway interactions during antibody class switch recombination [Tri-C]


ABSTRACT: Antibody class switch recombination (CSR) requires the ligation of DNA breaks in transcribed and juxtaposed donor and acceptor switch (S) sequences (S-S synapsis) spaced 50-200 kb apart within the immunoglobulin heavy chain locus (Igh). CSR is believed to occur via the association of active S regions with the 3’ regulatory region (3’RR) super-enhancer thereby coupling transcription with S-S synapsis. Here, we map the multi-way interactome underlying CSR using Tri-C. Surprisingly, S-S synapsis is strongly associated with the 3` CTCF binding element (3`CBE), but not the 3’RR, a finding also confirmed by Micro-C measurements. Instead, the 3’RR forms a self-interacting domain independently engaging with Igh genes. Additionally, transcription at acceptor S regions creates a topological barrier that insulates S-S synapsis from loop extrusion complexes. Thus, Igh gene activation and S-S synapsis occur via distinct topological states created by the interaction between transcription and the loop extrusion machinery.

ORGANISM(S): Mus musculus

PROVIDER: GSE198179 | GEO | 2023/01/20

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2023-01-20 | GSE198181 | GEO
2023-01-20 | GSE198180 | GEO
2023-01-20 | GSE198176 | GEO
2019-09-25 | GSE130266 | GEO
2019-09-25 | GSE130265 | GEO
2019-09-25 | GSE130264 | GEO
2019-09-25 | GSE130263 | GEO
2018-10-04 | GSE118794 | GEO
2016-02-17 | E-GEOD-62969 | biostudies-arrayexpress
2016-02-17 | GSE62969 | GEO