Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Investigation of the RNMT/RAM RNA interactome in mouse embryonic stem cells [Brain_seCLIP]

ABSTRACT: Pilot investigation to determine the endogenouse RNA-binding targets of the cap methlytransferase complex RNMT/RAM in mouse ES cells

ORGANISM(S): Mus musculus

PROVIDER: GSE198579 | GEO | 2025/03/14

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Investigation of the RNMT/RAM RNA interactome in mouse embryonic stem cells

Project description:Pilot investigation to determine the endogenouse RNA-binding targets of the cap methlytransferase complex RNMT/RAM in mouse ES cells

2025-03-11 | GSE198543 | GEO

Investigation of the RNMT/RAM RNA interactome in mouse embryonic stem cells [RIP-Seq]

Project description:Pilot investigation to determine the endogenouse RNA-binding targets of the cap methlytransferase complex RNMT/RAM in mouse ES cells

2025-03-18 | GSE198965 | GEO

mRNA cap methyltransferase, RNMT-RAM, promotes RNA pol II transcription

Project description:mRNA cap addition occurs early during RNA pol II transcription, facilitating pre-mRNA processing and translation. We report that the mammalian mRNA cap methyltransferase, RNMT-RAM, promotes RNA pol II transcription, independently of mRNA capping and translation. In cells, sub-lethal suppression of RNMT-RAM reduces RNA pol II occupancy, net mRNA synthesis and pre-mRNA levels. Conversely, expression of RNMT-RAM increases transcription independently of cap methyltransferase activity. In isolated nuclei, recombinant RNMT-RAM stimulates transcriptional output; this requires the RAM RNA-binding domain. RNMT-RAM interacts with nascent transcripts along their entire length and with transcription associated factors including RNA pol II subunits, SPT4, SPT6 and PAFc. Suppression of RNMT-RAM inhibits transcriptional markers including histone H2B K120 ubiquitination, H3 K4 and K36 methylation, RNA pol II S5 and S2 phosphorylation and PAFc recruitment. These findings suggest that multiple interactions between RNMT-RAM, RNA pol II factors and RNA along the transcription unit stimulate transcription.

2018-05-01 | GSE87767 | GEO

Rnmt and Ram regulate the activated CD4 T cell transcriptome

Project description:We investigated the role of RNMT and its cofactor RAM in T cells using conditional knockout mouse models. We demonstrate that RNMT and RAM have broadly overlapping roles in CD4 T cell activation.

2024-04-08 | GSE198541 | GEO

Investigation of the RNMT/RAM RNA interactome in mouse embryonic stem cells

Project description:Investigation of the RNMT/RAM RNA interactome in mouse embryonic stem cells

| PRJNA815852 | ENA

Investigation of the RNMT/RAM RNA interactome in mouse embryonic stem cells [Brain_seCLIP]

Project description:Investigation of the RNMT/RAM RNA interactome in mouse embryonic stem cells [Brain_seCLIP]

| PRJNA816065 | ENA

Investigation of the RNMT/RAM RNA interactome in mouse embryonic stem cells [RIP-Seq]

Project description:Investigation of the RNMT/RAM RNA interactome in mouse embryonic stem cells [RIP-Seq]

| PRJNA817589 | ENA

RNMT regulates the naïve CD4 T cell transcriptome

Project description:We investigated the role of RNMT in T cells using an Rnmt conditional knockout mouse model. We report that the mRNA cap methyltransferase, RNMT, supports naïve T cell survival and activation-induced proliferation. We demonstrate that RNMT has gene-specific impacts in T cells, selectively regulating expression of terminal polypyrimidine tract (TOP) mRNAs which are targets of the m7G-cap binding protein LARP1. These RNAseq experiments investigate the effect of Rnmt cKO on the naïve CD4 T cell transcriptome.

2021-05-13 | GSE160267 | GEO

Rnmt and Ram regulate the activated CD4 T cell transcriptome

Project description:Rnmt and Ram regulate the activated CD4 T cell transcriptome

| PRJNA815845 | ENA

RNMT regulates the activated CD4 T cell transcriptome and translatome [Ribo-Seq]

Project description:We investigated the role of RNMT in T cells using an Rnmt conditional knockout mouse model. We report that the mRNA cap methyltransferase, RNMT, supports naïve T cell survival and activation-induced proliferation. We demonstrate that RNMT has gene-specific impacts in T cells, selectively regulating expression of terminal polypyrimidine tract (TOP) mRNAs which are targets of the m7G-cap binding protein LARP1. These ribosome footprinting experiments investigate the effect of Rnmt cKO on the activated CD4 T cell transcriptome and translatome.

2021-05-13 | GSE160326 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data