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Early growth response 1 transcription factor is essential for the pathogenic properties of human endometriotic epithelial cells

ABSTRACT: The aim of this study is to compare transcriptome profiling of EGR1 siRNA treated immortalized human endometriotic epithelial cells expression luciferase (iHEECs/Luc) by RNA-sequencing Methods: The mRNA profiles of non-targeting (NT) and EGR1 siRNA treated immortalized human endometriotic epithelial cells expression luciferase (iHEECs/Luc) were generated by deep sequencing, in triplicate using Illumina NovaSeq-6000 sequencers with 2×100 paired-end reads. Basecalls and demultiplexing were performed with Illumina’s RTA version 1.9 and bcl2fastq2 software with a maximum of one mismatch in the indexing read. RNA-seq reads were then aligned to the human genome (Genome Reference Consortium Human Build hg38) with STAR version 2.5.1a. qRT–PCR validation was performed using TaqMan assays. Results: Using a 1.5-fold cutoff and Benjamini-Hochberg false discovery rate (FDR) of <0.05 threshold for inclusion, we identified 76 differentially expressed genes (DEGs) between (NT) and EGR1 siRNA treated immortalized human endometriotic epithelial cells. Conclusions: Our study represents the first detailed analysis of EGR1 siRNA treated immortalized human endometriotic epithelial cells expression luciferase (iHEECs/Luc) transcriptomes, with biologic replicates, generated by RNA-seq technology.

ORGANISM(S): Homo sapiens

PROVIDER: GSE199526 | GEO | 2022/11/15

REPOSITORIES: GEO

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