Transcriptomics

Dataset Information

0

Gut microbiota-derived short-chain fatty acids protect against the progression of endometriosis


ABSTRACT: The goal of this study is to compare transcriptome profiling of n-butyrate treated immortalized human endometriotic epithelial cells expression Luciferase (iHEECs/Luc) by RNA-sequencing Methods: The mRNA profiles of vehicle and n-butyrate treated immortalized human endometriotic epithelial cells expression Luciferase (iHEECs/Luc) were generated by deep sequencing, in triplicate using Illumina NovaSeq-6000 sequencers with 2×150 paired-end reads. Basecalls and demultiplexing were performed with Illumina’s RTA version 1.9 and bcl2fastq2 software with a maximum of one mismatch in the indexing read. RNA-seq reads were then aligned to the Ensembl release 76 primary assembly with STAR version 2.5.1a. qRT–PCR validation was performed using TaqMan assays. Results: Using a 2.5-fold cutoff and Benjamini-Hochberg false discovery rate (FDR) of <0.01 threshold for inclusion, we identified 1,830 differentially expressed genes (DEGs) between Vehicle and n-butyrate treated immortalized human endometriotic epithelial cells expression Luciferase (iHEECs/Luc). Conclusions: Our study represents the first detailed analysis of n-butyrate treated immortalized human endometriotic epithelial cells expression Luciferase (iHEECs/Luc) transcriptomes, with biologic replicates, generated by RNA-seq technology.

ORGANISM(S): Homo sapiens

PROVIDER: GSE184431 | GEO | 2021/09/29

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2022-11-15 | GSE199526 | GEO
2024-03-06 | GSE249959 | GEO
2018-03-07 | GSE111496 | GEO
2022-06-15 | GSE202661 | GEO
2011-03-31 | E-GEOD-18091 | biostudies-arrayexpress
2011-03-31 | E-GEOD-18094 | biostudies-arrayexpress
2023-09-01 | GSE236573 | GEO
2011-03-31 | E-GEOD-18093 | biostudies-arrayexpress
2019-11-19 | GSE136340 | GEO
2013-12-27 | GSE53651 | GEO