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CryoGrid-PIXUL-RNA: High throughput RNA isolation platform for tissue transcript analysis

ABSTRACT: We compared proteinase K buffer (PKBuffer) vs TRIzol vs column-based (PureLink RNA Micro kit) RNA isolation on frozen mouse organs methods using the high throughput CryoGrid system integrated with PIXUL sonicator. Mice were treated with intraperiteal injection of LPS (model of endotoxin induced sepsis) or PBS (control). After 12 hours mice were euthanized and brains, hearts, kidneys, and livers were harvested, immediately placed on ice, and then frozen in CryoTrays.

ORGANISM(S): Mus musculus

PROVIDER: GSE199598 | GEO | 2022/03/31

REPOSITORIES: GEO

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