Transcriptomics

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RNA-seq of bone marrow neutrophil treated PGE2 or Vehicle


ABSTRACT: Purpose: Investigate the neutrophils could exspress different mRNA de novo from PGE2 stimulation. Methods: Isolated bone marrow neutrophils with Percoll gradient are treated with 10 μM of PGE2 for 24 hours. Results: Qualified sequence reads per sample to the mouse genome (mm10) with Bowtie2 or STAR were processed with StringTie and identified genes. Conclusion : 1,359 genes were significantly different in PGE2 treated bone marrow neutrophils from vehicle treated ones.

ORGANISM(S): Mus musculus

PROVIDER: GSE199773 | GEO | 2022/08/01

REPOSITORIES: GEO

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