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The invadopodial and cytoskeletal regulator glycine- and cysteine-rich protein 2 induces pro-metastatic matrix metalloproteinase expression in breast cancer cells

ABSTRACT: Actin cytoskeleton has multiple functions in cancer cell invasion and metastasis; yet how these functions are coordinated remains unclear. In the cytoplasm, actin is necessary for the formation of specialized membrane protrusions, termed invadopodia, that concentrate and secrete extracellular matrix-degrading enzymes, particularly the matrix metalloproteinases (MMPs), facilitating tumor cell invasion. We recently identified cysteine- and glycine-rich protein 2 (CRP2) as an actin-crosslinking protein whose activity is required for the assembly and elongation of the invadopodium actin backbone. Here, we report that CRP2 is also located in the nucleus of breast cancer cells to induce a complementary pro-metastasis gene expression program mostly related to breast cancer cell invasion and metastasis. We showed in this present study that CRP2 interacts with the transcription factors SRF and GATA3 to regulated the expression of MMP-9 and MMP-13 respectively. Overall, our data indicate that CRP2 act a key mediator of tumor cell invasion.

ORGANISM(S): Homo sapiens

PROVIDER: GSE199822 | GEO | 2023/04/26

REPOSITORIES: GEO

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