Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome dysregulation in hepatoblastomas: the pivotal role of noncoding RNAs and detection of altered lipid metabolism

ABSTRACT: We report the application of the RNA sequencing technology for profiling gene expression alterations in hepatoblastomas. Comparing 14 hepatoblastomas to a group of control liver samples, a set of 1492 differentially expressed genes (DEGs) was detected, both coding and non-coding. We describe the enrichment of specific biological processes related to up- and downregulated genes, and the disruption of metabolic pathways including lipids, amines, and nicotinamides. Finally, we disclose a robust ncRNA transcriptome perturbation, highlighting a new epigenetic player in the control of gene expression in hepatoblastomas.

ORGANISM(S): Homo sapiens

PROVIDER: GSE199888 | GEO | 2022/04/03

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptome dysregulation in hepatoblastomas: the pivotal role of noncoding RNAs and detection of altered lipid metabolism

Project description:Transcriptome dysregulation in hepatoblastomas: the pivotal role of noncoding RNAs and detection of altered lipid metabolism

| PRJNA821888 | ENA

Spatial transcriptomics of primary human hepatoblastomas

Project description:Spatial transcriptomics was performed on human hepatoblastomas to determine features associated with histologic heterogeneity.

2024-09-12 | GSE249965 | GEO

DNA methylation landscape of hepatoblastomas reveals arrest at early stages of liver differentiation and cancer-related alterations

Project description:Hepatoblastomas carry few genetic alterations, and we hypothesize that epigenetic changes could be relevant to their onset. DNA methylation profile of hepatoblastomas was explored in relation to liver development using the HM450K platform. Seven paired samples of hepatoblastomas and adjacent non-tumoral livers were studied, with biologicaland results validation validated in an independent group (12 hepatoblastomas) that confirmed 1,359 differentiated methylated CpG sites (DMSs) in hepatoblastomas compared to controls, associated with 979 genes. Hepatoblastomas also exhibited a global low-level hypomethylation when compared with differentiated livers, especially at non-repetitive intergenic DNA (~55% of the hypomethylated CpGs); conversely, most of the hypermethylated CpGs were located in CpG islands. Functional analyses revealed an enrichment in signaling pathways involved in metabolism, negative regulation of cell differentiation, liver development, cancer, and the WNT pathway. Strikingly, an important overlap was observed between hepatoblastomas DMSs and the CpG sites reported to exhibit methylation changes through liver development. Genes with differential methylation were related to maintenance of undifferentiated cell state, cell transformation and tumor progression, and the methylation profile of tumors resembled that of fetal livers. Altogether, our results suggest an arrest at early stages of liver cell differentiation, in line with the hypothesis that hepatoblastoma ontogeny involves the disruption of liver development.

2017-04-03 | GSE78732 | GEO

Spatial transcriptomics of primary human hepatoblastomas

Project description:Spatial transcriptomics of primary human hepatoblastomas

| PRJNA1051513 | ENA

Hepatoblastomas with carcinoma features represent a biological spectrum of aggressive neoplasms in children and young adults

Project description:We molecularly characterized a class of histologically aggressive childhood liver cancers and showed that these tumors are clinically aggressive and that their observed histological features are associated with underlying recurrent molecular features. We proposed a diagnostic algorithm to identify these cancers using a combination of histological and molecular features, and our analysis suggested that these cancers may benefit from specialized treatment strategies that may differ from treatment guidelines for hepatoblastomas and hepatocellular carcinomas.

2022-07-15 | GSE208217 | GEO

Hepatoblastomas with carcinoma features represent a biological spectrum of aggressive neoplasms in children and young adults II

2022-09-24 | GSE214074 | GEO

Patient-Derived Mutant Forms of NFE2L2/NRF2 Drive Aggesssive Murine Hepatoblastomas

Project description:Patient-Derived Mutant Forms of NFE2L2/NRF2 Drive Aggesssive Murine Hepatoblastomas

| PRJNA662245 | ENA

FA-SAT ncRNA interactome

Project description:FA-SAT is a satellite DNA sequence present and transcribed in many Bilateria species, what may anticipate a conserved and significant function in these genomes. Here we prove that in cat and human cells, FA-SAT satellite transcripts play a nuclear function at the G1 phase of the cell cycle. We identified and demonstrated that the main FA-SAT non-coding RNA interactor is the PKM2 protein. Our work shows that the disruption of the FA-SAT ncRNA/PKM2 protein complex, by the depletion of either FA-SAT or PKM2, results in the same phenotype—apoptosis. Moreover, the ectopic overexpression of FA-SAT in tumour human cells did not affect the cell cycle progression. In sum, our data reveal a new player, FA-SAT RNA, a non-coding satellite RNA, which interacts with the PKM2 nuclear protein. This ribonucleoprotein is involved in apoptosis and cell cycle progression, what foresees a promising new target for studies in cancer processes that rely on these pathways.

2020-03-19 | PXD010081 | Pride

Dysregulation of Connexin Expression Plays a Pivotal Role in Psoriasis.

Project description:Psoriasis, a chronic inflammatory disease affecting 2-3% of the population, is characterised by epidermal hyperplasia, a sustained pro-inflammatory immune response and is primarily a T-cell driven disease. Previous work determined that Connexin26 is upregulated in psoriatic tissue. This study extends these findings. Biopsies spanning psoriatic plaque (PP) and non-involved tissue (PN) were compared to normal controls (NN). RNA was isolated and subject to real-time PCR to determine gene expression profiles, including GJB2/CX26, GJB6/CX30 and GJA1/CX43. Protein expression was assessed by immunohistochemistry. Keratinocytes and fibroblasts were isolated and used in 3D organotypic models. The pro-inflammatory status of fibroblasts and 3D cultures was assessed via ELISA and RnD cytokine arrays in the presence or absence of the connexin channel blocker Gap27. Connexin26 expression is dramatically enhanced at both transcriptional and translational level in PP and PN tissue compared to NN (>100x). In contrast, CX43 gene expression is not affected, but the protein is post-translationally modified and accumulates in psoriatic tissue. Fibroblasts isolated from psoriatic patients had a higher inflammatory index than normal fibroblasts and drove normal keratinocytes to adopt a "psoriatic phenotype" in a 3D-organotypic model. Exposure of normal fibroblasts to the pro-inflammatory mediator peptidoglycan, isolated from Staphylococcus aureus enhanced cytokine release, an event protected by Gap27. dysregulation of the connexin26:43 expression profile in psoriatic tissue contributes to an imbalance of cellular events. Inhibition of connexin signalling reduces pro-inflammatory events and may hold therapeutic benefit.

| S-EPMC8200029 | biostudies-literature

RNA sequencing of distinct histologic regions of primary human hepatoblastomas by Smart-3SEQ

Project description:RNA sequencing of distinct histologic regions of primary human hepatoblastomas by Smart-3SEQ

| PRJNA1172337 | ENA