Dataset Information

FA-SAT ncRNA interactome

ABSTRACT: FA-SAT is a satellite DNA sequence present and transcribed in many Bilateria species, what may anticipate a conserved and significant function in these genomes. Here we prove that in cat and human cells, FA-SAT satellite transcripts play a nuclear function at the G1 phase of the cell cycle. We identified and demonstrated that the main FA-SAT non-coding RNA interactor is the PKM2 protein. Our work shows that the disruption of the FA-SAT ncRNA/PKM2 protein complex, by the depletion of either FA-SAT or PKM2, results in the same phenotype—apoptosis. Moreover, the ectopic overexpression of FA-SAT in tumour human cells did not affect the cell cycle progression. In sum, our data reveal a new player, FA-SAT RNA, a non-coding satellite RNA, which interacts with the PKM2 nuclear protein. This ribonucleoprotein is involved in apoptosis and cell cycle progression, what foresees a promising new target for studies in cancer processes that rely on these pathways.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human) Bos Taurus (bovine) Felis Silvestris

SUBMITTER: Sandra Anjo

LAB HEAD: Bruno Manadas

PROVIDER: PXD010081 | Pride | 2020-03-19

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Database.fasta	Fasta
	ID136_FDR.txt	Txt
	ID136_IDA_sample1_fraction_1_10uL.wiff	Wiff
	ID136_IDA_sample1_fraction_1_10uL.wiff.scan	Wiff
	ID136_IDA_sample1_fraction_2_10uL.wiff	Wiff

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Publications

FA-SAT ncRNA interacts with PKM2 protein: depletion of this complex induces a switch from cell proliferation to apoptosis.

Ferreira Daniela D Escudeiro Ana A Adega Filomena F Anjo Sandra I SI Manadas Bruno B Chaves Raquel R

Cellular and molecular life sciences : CMLS 20190725 7

FA-SAT is a highly conserved satellite DNA sequence transcribed in many Bilateria species. To disclose the cellular and functional profile of FA-SAT non-coding RNAs, a comprehensive experimental approach, including the transcripts location in the cell and in the cell cycle, the identification of its putative protein interactors, and silencing/ectopic expression phenotype analysis, was performed. FA-SAT non-coding RNAs play a nuclear function at the G1 phase of the cell cycle and the interactomic ...[more]

PMID: 31346634

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