Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression of ZNF335, RNPC3, CLP1 and LIN52 inducible knockouts in HeLa cells

ABSTRACT: From our pooled optical screen, we identified an unexpected role for ZNF335, a transcription factor, in spindle bipolarity. We also found that RNPC3, CLP1 or LIN52 are regulators of gene expression on various levels, displayed an unexpected, specific kinetochore defect. Here we generated individual doxycycline-inducible Cas9 cell lines expressing lentiviral-integrated sgRNAs targeting ZNF335, LIN52, CLP1, and RNPC3 to evaluate differential roles in gene expression regulation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE199901 | GEO | 2022/04/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression of ZNF335, RNPC3, CLP1 and LIN52 inducible knockouts in HeLa cells

Project description:Gene expression of ZNF335, RNPC3, CLP1 and LIN52 inducible knockouts in HeLa cells

| PRJNA821912 | ENA

Gene expression of DREAM complex-related inducible knockouts in HeLa cells

Project description:The DREAM complex and interacting proteins (e.g., BMYB and FOXM1) together control cell cycle-dependent gene expression. We identified an unexpected, specific kinetochore defect phenotype after depletion of a DREAM complex member (LIN52) in optical pooled CRISPR knockout screens of essential genes. Here we generated individual doxycycline-inducible Cas9 cell lines expressing lentiviral-integrated sgRNAs targeting LIN52, MYBL2, and FOXM1 to evaluate differential roles in gene expression regulaion.

2021-11-22 | GSE188631 | GEO

The Cdc14 phosphatase, Clp1, does not affect genome expression

Project description:Schizosaccharomyces pombe Clp1 is a Cdc14-family phosphatase that reverses mitotic Cdk1 phosphorylation. Despite evolutionary conservation, Clp1’s mammalian orthologs do not share this function. Rather, higher eukaryotic Cdc14 enzymes act in DNA repair, ciliogenesis, and gene regulation. To examine if Clp1 regulates gene expression, we compared the transcriptional profiles of cells lacking Clp1 function to that of wildtype. Because clp1∆ cells are sensitive to the actin depolymerizing drug, LatrunculinA, we also investigated whether a transcriptional response was involved. Our results indicate that Clp1 does not detectably affect gene expression and highlight the organism-specific functions of this conserved phosphatase family.

2024-03-11 | GSE255124 | GEO

Effect of clp1 induction on rbf1-dependent gene regulation

2010-09-17 | GSE21121 | GEO

Clp1 knockdown lentigenic mouse generation

Project description:Clp1 promotes 3’UTR shortening and higher levels of Aire-upregulated transcripts in mTEChi

2020-05-05 | GSE140878 | GEO

Effect of clp1 induction on rbf1-dependent gene regulation

Project description:The clp1 gene is a b-regulated gene that is required for clamp formation during the biotrophic growth phase of U. maydis. Interaction studies demonstrated that Clp1 imnteracts with Rbf1, the central regulator of pathogenciity. To exclude b-dependent effects the experiments were performed in a b-null background to analyze clp1-depndent effects on rbf controlled gene regulation Strains UKH156 (nitrate inducible Rbf1 expression) and UKH164 (arabinose-inducible Clp1 and and nitrate-inducible Rbf1 expression) were grown for 12h in liquid array medium/nitrate/arabinose.

2010-09-17 | E-GEOD-21121 | biostudies-arrayexpress

Genomewide Analysis of Clp1 Function in Transcription in Budding Yeast

Project description:We report the GRO-Seq analysis in Clp1 mutant and the isogenic wild type strain to determine the the role of Clp1 in termination of transcription on a genomewide scale.

2017-07-31 | GSE71624 | GEO

Effect of clp1 induction on b-dependent gene regulation

2011-03-30 | GSE21119 | GEO

clp1 induction

Project description:The clp1 gene is a b-dependent gene required for clamp formation during the biotrophic growth phase of U. maydis. The gene was induced by means of a fusion to the arabinose inducable crg1 promoter. To exclude effects from the b-mating type locus, the experiments were performed in strain JB1 carrying a deletion of the entire b-locus. Keywords: gene induction

2006-04-25 | GSE4689 | GEO

Effect of clp1 induction on b-dependent gene regulation

Project description:The clp1 gene is a b-regulated gene that is required for clamp formation during the biotrophic growth phase of U. maydis. Clp1 expression blocks b-dependent filament formation and cell cycle arrest if co-induced with the b-heterodimer. Strains AB33 (control) and UMS84 were grown for the times indicated in array medium containing 3 g/l nitrate and 1% arabinose for the induction of nar1P- (bE1/bW2) and crg1P- (clp1) controlled gene expression. Samples were taken from two biological replicates each.

2011-03-30 | E-GEOD-21119 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data