RNA-seq analysis in roots of PuHox52 overexpression and wild type Populus ussuriensis under normal nitrogen and low nitrogen condition.
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ABSTRACT: To gain insight into the molecular mechanism of PuHox52-mediated low-N adaption, we performed a transcriptome sequencing analysis (RNA-seq) using the PuHox52-OE lines and WT P. ussuriensis in roots under low-N for 3 wks, as well as roots under normal-N as controls.
Project description:Drought is one of the major environmental problem in terms of limiting the survival of plant. Roots are the first plant organ to sense drought stress, so it is important to understanding of the molecular machanisms of drought stress responses in the roots. Here we aim to characterized the gene expression profile in Populus ussuriensis roots at 0, 6, 12, 24, 48 and 120 h after the start of PEG-induced drought stress. A total of 2 μg RNA per sample was used to generate sequencing libraries using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA). Libraries were sequenced in 150 bp paired-end mode, using an Illumina HiSeq X Ten platform. Our results provide a global view of gene expression profile that contributes to drought resistance in Populus ussuriensis, and meaningful information for genetic engineering research in the future.
Project description:One week-old in-vitro plants of PuCH35 overexpression (PuC3H35-OE) lines and wildtype (WT) Populus ussuriensis were transfered to 1/2 MS medium supplemented with or without 6% PEG6000 for 7 days, and the root tips (0.5 cm long) were harvested. Samples were collected from three biological replicates (25 in-vitro plants each).
Project description:RNA-seq analysis in roots of PuHox52 overexpression and wild type Populus ussuriensis under normal nitrogen and low nitrogen condition.
Project description:RNA-seq was performed to examine the differential expressed transcriptomes with five-point experiment (6, 12, 24, 48 and 96 h) at the stem bases of cuttings in PuHox52 overexpression line compare to wild type Populus ussuriensis.