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The osteogenic capacity and lncRNA/mRNA expression profile of periosteal-derived mesenchymal stem cells of congenital pseudarthrosis of the tibia

ABSTRACT: Purpose: Congenital pseudarthrosis of the tibia (CPT) is an uncommon disease. The biological properties, molecular patterns, and regulatory mechanisms of periosteal-derived mesenchymal stromal cells (PDMSCs) remain unclear. Methods: PDMSCs were separated from patients with either CPT or control donors. The purity of stem cells was determined by flow cytometry. Using osteogenic induction culture and Co-culture THP1-derived macrophage and PDMSCs, the osteogenic and osteoclastic activities were determined by histological examination and western blot analysis. The differential expression of lncRNA/mRNA was analyzed through RNA-Seq. Results: The proportion of CD31 and CD34 positive cells were less than 1.5%, while CD44 and CD90 positive cells were higher than 95% in PDMSCs. After osteogenic differentiation induction, the calcium nodule formation and osteogenic protein indicators levels were lower in CPT-PDMSCs. Co-culture THP1-derived macrophage and PDMSCs, after osteoclast differentiation induction, dissolved hydroxyapatite plate activity, osteoclastic protein indicators levels of co-culture with CPT PDMSCs were shown to be higher than control. RNA-seq results revealed 822 differentially expressed mRNAs and 194 differentially expressed lncRNAs, respectively. Functional analysis showed that differentially expressed lncRNAs involved in tryptophan metabolism, steroid biosynthesis, and so on. Based on differential mRNAs and lncRNAs, we conducted correlation analysis of their cis regulation, and the results showed that 27 mRNAs were cis regulated with 24 lncRNAs, a total of 30 cis-regulated combinations. Conclusion: CPT-PDMSCs has weak osteogenesis ability, but stronger ability to promotes osteoclast differentiation, we found that certain lncRNAs and mRNAs were differentially expressed in CPT-PDMSCs which have important roles in regulated osteogenesis and osteoclast differentiation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE200069 | GEO | 2022/09/20

REPOSITORIES: GEO

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The osteogenic capacity and lncRNA/mRNA expression profile of periosteal-derived mesenchymal stem cells of congenital pseudarthrosis of the tibia

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