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Peripheral blood circRNA expression profiles and circRNA-related functions in children with autism spectrum disorder: A preliminary analysis based on circRNA chips

ABSTRACT: The incidence rate of autism spectrum disorder (ASD) is increasing year by year. Apart from some knowledge of the clinical manifestations of ASD, there has been no significant progress in the study of its pathogenesis and biomarkers. To find biomarkers for early clinical diagnosis, we analyzed the expression profiles of circRNAs in peripheral blood of ASD children. After preliminary screening, among the 160985 circRNA sequences obtained, 461 differentially expressed circRNAs were identified, including 270 up-regulated ones and 146 down-regulated ones. The expression difference of 2 randomly selected circRNAs was verified by qPCR. Gene ontology (GO) and Kyoto Encyclopedia of genes and genomes (KEGG) pathway databases were used for enrichment analysis of biological functions. Dysregulated circRNAs were found abundant in biological functions related to synaptic structure, morphological development, and nervous system development. Pathway analysis suggested that extracellular matrix (ECM) receptor interaction, which is associated with synaptic plasticity and behavior, might be related to ASD. Our circRNA-microRNA interaction analysis showed that most circRNAs had multiple microRNAs binding sites, including miR-146, which might contribute to neuroinflammation of ASD. Our results show that some circRNAs are differently expressed in children with ASD and may take a part in the disease process. The differentially expressed circRNAs in peripheral blood have the potential to serve as biomarkers for early clinical screening of ASD.

ORGANISM(S): Homo sapiens

PROVIDER: GSE200197 | GEO | 2022/07/13

REPOSITORIES: GEO

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